Arginase 1/ARG1/liver Arginase Antibody

Western Blot: Arginase 1/ARG1/liver Arginase Antibody [NB100-59740] - Staining of Mouse Liver (A) and Rat Liver (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Simple Western: Arginase 1/ARG1/liver Arginase Antibody [NB100-59740] - Simple Western lane view shows a specific band for ARG1 in mouse Liver lysate using ARG1 antibody (NB100-59740) at 5 ug/ml. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Arginase 1/ARG1/liver Arginase Antibody Summary

• Immunogen: Peptide with sequence C-NHKPETDYLKPPK corresponding to C-Terminus according to Uniprot Rat NP_058830.2.
• Epitope: NHKPETDYLKPPK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.03 - 0.1 ug/ml
  • Simple Western 5 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA 1:128000
• Application Notes: WB: Approx. 37 kDa band observed in mouse and rat liver lysates (calculated MW of 34.8 kDa band according to mouse NP_031508.1). IHC-F usage is reported in scientific literature (PMID: 24089194). Use in ICC/IF reported in scientific literature (PMID 28246332). Use in FC reported in (PMID: 24089194). Use in IHC reported in scientific literature (PMID: 23207546).
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Rat Liver Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28246332).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Arginase 1/ARG1/liver Arginase Antibody (NB100-59740)(9)

Publications using NB100-59740

• Sadler R, Cramer JV, Heindl S et al. Short-Chain Fatty Acids Improve Poststroke Recovery via Immunological Mechanisms J. Neurosci. Jan 29 2020 [PMID: 31889008] (IHC, Mouse)
• Rodet F, Capuz A, Ozcan BA et al. PC1/3 KD Macrophages Exhibit Resistance to the Inhibitory Effect of IL-10 and a Higher TLR4 Activation Rate, Leading to an Anti-Tumoral Phenotype Cells Nov 22 2019 [PMID: 31766635] (WB, Rat)
• Carmona-Fontaine C, Deforet M, Akkari L et al. Metabolic origins of spatial organization in the tumor microenvironment. Proc. Natl. Acad. Sci. U.S.A. 2017 Feb 28 [PMID: 28246332] (ICC/IF, Human)
• Jang E, Kim JH, Lee S et al. Phenotypic polarization of activated astrocytes: the critical role of lipocalin-2 in the classical inflammatory activation of astrocytes. J Immunol. 2013 Nov 15 [PMID: 24089194] (FLOW, IHC-Fr, ICC/IF, Mouse)
• Jang E, Lee S, Kim JH et al. Secreted protein lipocalin-2 promotes microglial M1 polarization FASEB J 2012 Dec 4 [PMID: 23207546] (FLOW, IHC, ICC/IF, Mouse)
• Yu M, Eckart MR, Morgan AA et al. Identification of an IFN-gamma/mast cell axis in a mouse model of chronic asthma. J Clin Invest. 2011 Jul 1. [PMID: 21737883]
• Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O. All-trans retinoic acid modifies the expression of clock and disease marker genes. J Nutr Biochem. 2011 Apr 14. [PMID: 21497500]
• Sherman H, Gutman R, Chapnik N, Meylan J, le Coutre J, Froy O. Caffeine alters circadian rhythms and expression of disease and metabolic markers. Int J Biochem Cell Biol;43(5):829-38. 2011 May. [PMID: 21352949]
• Jiang M, Ding Y, Su Y et al. Arginase-flotillin interaction brings arginase to red blood cell membrane. FEBS Lett 2006 Dec 11 [PMID: 17113085]

Bioinformatics

• Gene Symbol: ARG1
• Entrez:
  • 11846(Mouse)
  • 29221(Rat)
• Uniprot:
  • P07824(Rat)