Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125)

Western Blot: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis A) Recombinant ARG1 Protein Fragment (B) human Liver Lysate using ARG1 Monoclonal Antibody (ARG1/1125).

Immunohistochemistry-Paraffin: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Human Hepatocellular Carcinoma stained with ARG1 Monoclonal Antibody (ARG1/1125).

Protein Array: Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) [NBP2-44965] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-P, PA, ICC/IF
• Clone: ARG1/1125
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125) Summary

• Immunogen: Recombinant fragment (87 Amino acid residues around aa 1-150) of human ARG1 protein
• Localization: Cytoplasmic
• Marker: Hepatocellular Carcinoma Marker
• Specificity: Recognizes a protein of 35-38kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma.
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARG1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 1.0 ug/ml
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Protein Array
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Hu-chromosome location: 6q23
  Molecular weight of antigen: 35-38kDa
  Immunohistochemistry-Paraffin 2 - 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 36.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Arginase 1/ARG1/liver Arginase Antibody (ARG1/1125)

• AI
• ARG1
• Arginase 1
• arginase, liver
• Arginase-1
• EC 3.5.3.1
• Liver Arginase
• Liver-type arginase
• PGIF
• Type I Arginase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARG1
• Uniprot:
  • P05089()