APRIN Antibody

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Western Blot: APRIN Antibody [NB100-755] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPds5b antibody ...read more
Immunohistochemistry: APRIN Antibody [NB100-755] - Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti- Pds5B used at a dilution of 1:200 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: APRIN Antibody [NB100-755] - Detection of Human and Mouse Pds5B on HeLa whole cell lysate using NB100-755. Pds5B was also immunoprecipitated using rabbit anti-Pds5B antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

APRIN Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1447) of human Precocious Dissociation of Sisters-5B using the numbering given in entry CAB69911.1 (GeneID 23047).
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDS5B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-755 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APRIN Antibody

  • Androgen-induced proliferation inhibitorKIAA0979androgen induced inhibitor of proliferation
  • Androgen-induced prostate proliferative shutoff-associated protein AS3
  • androgen-induced shutoff 3
  • AS3APRIN
  • CG008
  • FLJ23236
  • PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
  • sister chromatid cohesion protein PDS5 homolog B

Background

Pds5B is a cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. It plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for APRIN Antibody (NB100-755)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Bioinformatics

Gene Symbol PDS5B
Entrez
Uniprot