APRIL/TNFSF13 Antibody [Unconjugated]

Recombinant Human APRIL/TNFSF13 protein was serially diluted 2-fold and captured by Mouse Anti-Human APRIL/TNFSF13 Monoclonal Antibody (MAB8844) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

U937 human lymphoma cell line was stained with Goat Anti-Human APRIL/TNFSF13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF884, filled histogram) or control antibody staining (AB-108-C, open histogram) followed by anti-Goat IgG PE-conjugated Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

APRIL/TNFSF13 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human APRIL/TNFSF13
• Specificity: Detects human APRIL/TNFSF13 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Fas Ligand, rhTNF-alpha , rhTRAIL, and rhTRANCE is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APRIL/TNFSF13 Antibody [Unconjugated]

• A proliferation-inducing ligand
• APRIL
• APRILFLJ57090
• CD256 antigen
• CD256
• TALL2
• TALL-2
• TNF- and APOL-related leukocyte expressed ligand 2
• TNF-related death ligand 1
• TNFSF13
• TRDL1
• TRDL-1
• tumor necrosis factor (ligand) superfamily, member 13
• tumor necrosis factor ligand superfamily member 13
• tumor necrosis factor-like protein ZTNF2
• tumor necrosis factor-related death ligand-1
• ZTNF2

Background

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
7. He, B. et al. (2004) J. Immunol. 172:3268.
8. Schwaller, J. et al. (2007) Blood 109:331.
9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
10. Day, E.S. et al. (2005) Biochemistry 44:1919.
11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APRIL/TNFSF13 Antibody (AF884)(4)

Publications using AF884

• Wioletta Ratajczak-Wrona, Natalia Wawrusiewicz-Kurylonek, Marzena Garley, Adam Jacek Kretowski, Ewa Jablonska A Proliferation-Inducing Ligand Regulation in Polymorphonuclear Neutrophils by Panax ginseng Archivum Immunologiae et Therapiae Experimentalis 2020-12-01 [PMID: 33125603]
• H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Sci Rep, 2020-07-28;10(1):12572. 2020-07-28 [PMID: 32724091] (ICC, Western Blot, Human)
• Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012-09-17;209(10):1813-23. 2012-09-17 [PMID: 22987802] (Flow Cytometry, Human)
• Moreaux J, Legouffe E, Jourdan E, Quittet P, Reme T, Lugagne C, Moine P, Rossi JF, Klein B, Tarte K BAFF and APRIL protect myeloma cells from apoptosis induced by interleukin 6 deprivation and dexamethasone. Blood, 2003-12-04;103(8):3148-57. 2003-12-04 [PMID: 15070697] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: TNFSF13