APRIL/TNFSF13 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

APRIL/TNFSF13 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human APRIL
  aa 110-250
• Specificity: Detects human APRIL in Western blots. In this format, less than 1% cross-reactivity is observed with rhTRANCE, rhTNF-alpha , rhFas Ligand, rhTRAIL, rhGITR, and rhVEGI.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF13
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APRIL/TNFSF13 Antibody [Biotin]

• A proliferation-inducing ligand
• APRIL
• APRILFLJ57090
• CD256 antigen
• CD256
• TALL2
• TALL-2
• TNF- and APOL-related leukocyte expressed ligand 2
• TNF-related death ligand 1
• TNFSF13
• TRDL1
• TRDL-1
• tumor necrosis factor (ligand) superfamily, member 13
• tumor necrosis factor ligand superfamily member 13
• tumor necrosis factor-like protein ZTNF2
• tumor necrosis factor-related death ligand-1
• ZTNF2

Background

APRIL (a proliferation-inducing ligand), also known as TNFSF13, TALL2, and TRDL1, is a member of the TNF ligand superfamily (1). APRIL is synthesized as a 32 kDa type II transmembrane protein which is cleaved by furin in the Golgi to release a 17 kDa soluble molecule (2, 3). Secreted APRIL consists almost entirely of a single TNF homology domain (2, 3). Little or no transmembrane APRIL is expressed on the cell surface (3). Alternate splicing generates isoforms with short deletions at the N- or C-terminus (4). Human APRIL shares 85% aa sequence identity with mouse and rat APRIL. Among TNF superfamily ligands, BAFF shows the greatest sequence homology with APRIL, and the two proteins exhibit overlapping biological activities. APRIL promotes cellular proliferation and protects from apoptosis in normal and transformed cells (3, 5‑7). It is present in elevated amounts in a wide variety of cancers primarily due to expression by tumor-infiltrating neutrophils (4, 5, 7‑9). Both APRIL and BAFF bind and signal through the TNF superfamily receptors TACI and BCMA, and BAFF additionally functions through BAFF R (6, 10, 11). A stretch of basic amino acids at the N-terminus of APRIL is required for its interaction with heparan sulfate proteoglycans (HSPGs) (12, 13). Binding to HSPGs is independent of APRIL’s binding to TACI and BCMA (12, 13). Interaction with HSPGs serves to concentrate APRIL on the surface of cells, thereby favoring TACI- or BCMA-mediated effects (8, 9, 13). APRIL can form bioactive heterotrimers with BAFF, and these circulate in the serum of patients with rheumatic immune disorders (14). A bioactive protein known as TWE-PRIL consists of the intracellular domain, transmembrane segment, and stalk region of TWEAK fused to the TNF homology domain of APRIL (15). TWE-PRIL is expressed in monocytes and activated T cells, and in contrast to APRIL, it is presented on the cell surface (15).

1. Planelles, L. et al. (2008) Curr. Mol. Med. 8:829.
2. Hahne, M. et al. (1998) J. Exp. Med. 188:1185.
3. Lopez-Fraga, M. et al. (2001) EMBO Reports 2:945.
4. Kelly, K. et al. (2000) Cancer Res. 60:1021.
5. Roth, W. et al. (2001) Cell Death Differ. 8:403.
6. Yu, G. et al. (2000) Nat. Immunol. 1:252.
7. He, B. et al. (2004) J. Immunol. 172:3268.
8. Schwaller, J. et al. (2007) Blood 109:331.
9. Mhawech-Fauceglia, P. et al. (2008) Eur. J. Cancer 44:2097.
10. Day, E.S. et al. (2005) Biochemistry 44:1919.
11. Bossen, C. et al. (2006) J. Biol. Chem. 281:13964.
12. Hendriks, J. et al. (2005) Cell Death Differ. 12:637.
13. Ingold, K. et al. (2005) J. Exp. Med. 201:1375.
14. Roschke, V. et al. (2002) J. Immunol. 169:4314.
15. Pradet-Balade, B. et al. (2002) EMBO J. 21:5711.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APRIL/TNFSF13 Antibody (BAF884)(2)

Publications using BAF884

• M Suzuki, T Sujino, S Chiba, Y Harada, M Goto, R Takahashi, M Mita, K Hamase, T Kanai, M Ito, MK Waldor, M Yasui, J Sasabe Host-microbe cross-talk governs amino acid chirality to regulate survival and differentiation of B cells Science Advances, 2021-03-03;7(10):. 2021-03-03 [PMID: 33658193] (Western Blot, Mouse)
• He B, Xu W, Santini PA Intestinal bacteria trigger T cell-independent immunoglobulin A(2) class switching by inducing epithelial-cell secretion of the cytokine APRIL. Immunity, 2007-06-01;26(6):812-26. 2007-06-01 [PMID: 17570691] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: TNFSF13