APP Antibody (Y188)

Product Discontinued

APP Antibody (Y188) Summary

• Immunogen: A synthetic peptide corresponding to residues in the NPXY motif of human Amyloid beta precursor protein was used as immunogen.
• Specificity: This antibody specifically recognizes Amyloid beta precursor protein and its isoforms. It will not recognize either of the two Amyloid Beta Protein ( ABeta4 ) variants, ABeta1 -40 or ABeta1 -42.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: APP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:250-500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:750
  • Immunohistochemistry-Paraffin 1:250-500
  • Immunoprecipitation 1:50
  • Simple Western
  • Western Blot 1:100-10000
• Application Notes: In Western blot this antibody detects a band at approximately 95kDa. Use in IHC-F was reported in the scientific literature (PMID: 18974297).
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for APP Antibody (Y188)

• amyloid beta (A4) precursor protein-binding, family B, member 2
• amyloid beta A4 precursor protein-binding family B member 2
• Amyloid beta precursor protein
• Amyloid beta
• APP
• beta Amyloid
• Protease Nexin II

Background

Amyloid peptides, derived from amyloid precursor protein (APP), are thought to play a role in the development of the senile plaques associated with Alzheimer's Disease. The amyloid hypothesis presupposes that flaws in the processing of APP result in abnormally high levels of the longer, "stickier" forms of beta amyloid, known as ABeta42 and ABeta43, leading to aggregation of amyloid in the neuronal cell death and ultimately neuronal death. Mutations in the structure of ABeta40 and related peptides as well as in some of the enzymes involved in the processing of APP have been shown to alter the processing of APP. The sporadic (i.e., non-genetic) form of the disease, however, is far more common, caused by aging in concert with a number of both genetic and environmental risk factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APP Antibody (NB110-55461)(4)

Publications using NB110-55461

• Hwang CJ, Yun HM, Park KR et al. Memory Impairment in Estrogen Receptor a Knockout Mice Through Accumulation of Amyloid-b Peptides. Mol Neurobiol. 2014-08-17 [PMID: 25128029] (WB, Mouse)
• Barbagallo AP, Wang Z, Zheng H et al. A single tyrosine residue in the amyloid precursor protein intracellular domain is essential for developmental function. J Biol Chem 2011-03-01 [PMID: 21266574]
• Li H, Wang B, Wang Z et al. Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP . Proc Natl Acad Sci U S A 107 17362-17367. 2010-01-01 [PMID: 20855613]
• Wang Z, Wang B, Yang L et al. Presynaptic and postsynaptic interaction of the amyloid precursor protein promotes peripheral and central synaptogenesis. J Neurosci 2009-09-01 [PMID: 19726636]

Reviews for APP Antibody (NB110-55461) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 review tested in 1 species: Mouse.

reviewed by: Jesse Alt WB 08/01/2014

Summary

• Application: Western Blot
• Lot: gr149051-1

reviewed by: Kui Xu IP Mouse 07/02/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: Mice Brain Lysate
• Species: Mouse
• Lot: YG021807C GR89483-9 YJ021703CS

Bioinformatics

• Gene Symbol: APP
• Entrez:
  • 351(Human)
  • 11820(Mouse)
  • 54226(Rat)
• Uniprot:
  • P05067()