APP Antibodies

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human brain (Alzheimer's disease cortex) using Goat Anti-Human APP/Protease Nexin II Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1168) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of L-929 mouse fibroblast cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-APP/Protease Nexin II (T668) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2508), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="APP/Protease Nexin II was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 8 µg/mL Human/Mouse Phospho-APP/Protease Nexin II (T668) Monoclonal Antibody (Catalog # MAB2508) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0135) and Mouse anti-Human CD14 PE-conjugated antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0135) and Mouse anti-Human CD14 PE-conjugated antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Human Anti-Human APP/Protease Nexin II (Aducanumab Biosimilar) Monoclonal Antibody (Catalog # MAB9710) or (B) no primary antibody, followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD14 PE-conjugated antibody (Catalog # FAB3832P). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

MAB96181or Catalog # MAB96181R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB96181or Catalog # MAB96181R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Amyloid beta protein was serially diluted 2-fold and captured by Mouse Anti-Human Amyloid beta (aa1-40) Monoclonal Antibody (Catalog # MAB96181or Catalog # MAB96181R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

MAB9618or Catalog # MAB9618R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB9618or Catalog # MAB9618R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human APP/Protease Nexin II (Amyloid beta) Monoclonal Antibody (Catalog # MAB96182) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />