Apolipoprotein A-IV/ApoA4 Antibody [Unconjugated]

Images

 
Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by ...read more
Western blot shows mouse plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A‑IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by ...read more
Apolipoprotein A‑IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Sheep Anti-Human/Mouse Apolipoprotein A-IV/ApoA4 Antigen Affinity-purified Polyclonal ...read more
Simple Western lane view shows human plasma, loaded at 0.5 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse ...read more
Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human ileum (left panel) and descending colon (right panel) using Sheep Anti-Human/Mouse Apolipoprotein A‑IV/ApoA4 Antigen ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Apolipoprotein A-IV/ApoA4 Antibody [Unconjugated] Summary

Immunogen
E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA
Glu21-Ser396
Accession # P06727
Specificity
Detects human Apolipoprotein A-IV/ApoA in direct ELISAs. Detects human and mouse in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
APOA4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2-2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 1-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Apolipoprotein A-IV/ApoA4 Antibody [Unconjugated]

  • apo-AIV
  • apoA-IV
  • Apolipoprotein A4
  • Apolipoprotein AIV
  • Apolipoprotein A-IV
  • MGC142154
  • MGC142156

Background

Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system.  Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption.  Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis.  ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well.  Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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FAQs for Apolipoprotein A-IV/ApoA4 Antibody (AF8125). (Showing 1 - 1 of 1 FAQ).

  1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
    • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.

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Bioinformatics

Gene Symbol APOA4
Uniprot