gamma Sarcoglycan Antibody

Immunohistochemistry-Paraffin: gamma Sarcoglycan Antibody [NBP1-90299] - Staining in human heart muscle and prostate tissues using anti-SGCG antibody. Corresponding SGCG RNA-seq data are presented for the same tissues.

Western Blot: gamma Sarcoglycan Antibody [NBP1-90299] - Analysis in control (vector only transfected HEK293T lysate) and SGCG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: gamma Sarcoglycan Antibody [NBP1-90299] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry-Frozen: gamma Sarcoglycan Antibody [NBP1-90299] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: gamma Sarcoglycan Antibody [NBP1-90299] - Staining of human heart muscle shows high expression.

Immunohistochemistry-Paraffin: gamma Sarcoglycan Antibody [NBP1-90299] - Staining of human prostate shows low expression as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

gamma Sarcoglycan Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGCG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Gamma Sarcoglycan antibody validated for IHC-Fr from a verified customer review.
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  gamma Sarcoglycan Protein (NBP1-90299PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for gamma Sarcoglycan Antibody

• 35 kDa dystrophin-associated glycoprotein
• 35DAG
• A4
• DAGA4
• DAGA4,35kD dystrophin-associated glycoprotein
• DMDA
• DMDA1
• gamma sarcoglycan
• gamma-Sarcoglycan
• gamma-SG
• LGMD2C
• LGMDR5
• MAM
• MGC130048
• sarcoglycan, gamma (35kD dystrophin-associated glycoprotein)
• sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
• SCARMD2
• SCG3
• SCG3LGMD2C
• TYPE

Background

gamma Sarcoglycan encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for gamma Sarcoglycan Antibody (NBP1-90299) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 10/08/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Skeletal muscle tissue
• Species: Human
• Lot: A57738

Bioinformatics

• Gene Symbol: SGCG
• Entrez:
  • 6445(Human)
• Uniprot:
  • Q13326(Human)