Apolipoprotein A-IV/ApoA4 Antibody [Alexa Fluor® 750]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Apolipoprotein A-IV/ApoA4 Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA
  Glu21-Ser396
  Accession # P06727
• Specificity: Detects human Apolipoprotein A-IV/ApoA in direct ELISAs. Detects human and mouse in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system.  Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption.  Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis.  ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well.  Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Apolipoprotein A-IV/ApoA4 Antibody (AF8125S). (Showing 1 - 1 of 1 FAQ).

1. I would like to buy antibodies APOA4 (apolipoprotein AIV) for the cows (to western blot). I know that You don't have the antibodies for this species. Could You propose the best atibodies which I could test (use) in my experiment?
   • All eight of our APOA4 antibodies were raised against the human protein, and all of them have currently only been tested against human samples. I ran an alignment for you and found that there is a 77% sequence homology between human and bovine APOA4, so it is possible that the antibodies raised against human APOA4 may recognise the bovine protein. Should you wish to try any of our antibodies with your samples, you may be interested in our Innovator's Reward Program, which allows you to try our primary antibodies in an untested species or application without the financial risk of failure. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product.