APMAP Antibody - BSA Free

Western Blot: APMAP Antibody [NBP3-12576] - Lane A: MDA-MB-231 Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:50 kDa

Immunohistochemistry-Paraffin: APMAP Antibody [NBP3-12576] - Staining of human C20orf3 in human liver with rabbit polyclonal antibody at 1:500 dilution.

Immunoprecipitation: APMAP Antibody [NBP3-12576] - Lane A:0.5 mg MDA-MB-231 Whole Cell Lysate4 uL anti-C20orf3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-C20orf3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :50 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

APMAP Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human APMAP fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APMAP
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for APMAP Antibody - BSA Free

• adipocyte plasma membrane-associated protein
• APMAP
• BSCv
• chromosome 20 open reading frame 3
• Protein BSCv

Background

Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role inadipocyte differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APMAP