dilution 1:10 in 1% bovine serum albumin in PBS, pH 7.4, incubation 1h at room temperature
donkey anti-rabbit Alexa 488
Secondary Manufacturer Cat#
1:1000 dilution in PBS
confocal LSM710 microscope from Carl Zeiss
fixation with 4% paraformaldehyde for 20 min and permeabilization by 0.2% Triton-X in PBS, pH 7.4, for 10 min
3 times for 5 minutes in PBS, pH 7.4
The antibody NBP2-13881 stained nicely NK92 cells and cystatin F transfected HEK293 cells, however it also stained non-transfected HEK293 that do not express cystatin F. Antigen retreival methods were not tested.
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