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APEX2 Antibody

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Western Blot: APEX2 Antibody [NB100-56625] - Western blot analysis of APEXL2 in HeLa cell lysates using NB100-56625 at a dilution of 1:500.
Western Blot: APEX2 Antibody [NB100-56625] - Western blot analysis of APEXL2 in HeLa cell lysates using NB100-56625 at a dilution of 1:500.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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APEX2 Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with EELRALDSEGRALLTQHKI (aa122-140) and GQAKTSEAKDEKELRTSF (aa438-455) peptides of human APEX2.
Specificity
In HeLa, a 54 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
APEX2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Lysate (NB800-PC1)
APEX2 Lysate (NBP2-65851)
Publications
Read Publication using NB100-56625.

Reactivity Notes

Predicted cross-reactivity based on sequence identity (not validated): Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for APEX2 Antibody

  • AP endonuclease 2
  • APE2
  • APEX nuclease (apurinic/apyrimidinic endonuclease) 2
  • APEX nuclease 2
  • APEXL2
  • Apurinic-apyrimidinic endonuclease 2
  • DNA-(apurinic or apyrimidinic site) lyase 2
  • DNA-apurinic or apyrimidinic site lyase 2
  • EC 3.1
  • EC 4.2.99.18
  • XTH2

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA by spontaneous hydrolysis, DNA damaging agents or DNA glycosylases that remove specific abnormal bases. ClassII AP endonucleases act to repair this damage by cleaving the phosphodiester backbone. It is primarily located in the nucleus but is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: APE Antibody (13B8E5C2) [NB100-116] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-APE (13B8E5C2) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Publications for APEX2 Antibody (NB100-56625)(1)

We have publications tested in 2 applications: ChIP, WB.


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Control Lysate(s)

HeLa Whole Cell Lysate
APEX2 Knockout 293T Cell Lysate

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Bioinformatics

Gene Symbol APEX2
Entrez
Uniprot