APEX2 Antibody - BSA Free

Western Blot: APEX2 Antibody [NB100-56625] - Western blot analysis of APEXL2 in HeLa cell lysates using NB100-56625 at a dilution of 1:500.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

APEX2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit APEX2 Antibody - BSA Free (NB100-56625) is a polyclonal antibody validated for use in WB. Anti-APEX2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated by immunizing rabbits with EELRALDSEGRALLTQHKI (aa122-140) and GQAKTSEAKDEKELRTSF (aa438-455) peptides of human APEX2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APEX2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Application Notes: In HeLa, a 54 kDa band is observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity (not validated): Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for APEX2 Antibody - BSA Free

• AP endonuclease 2
• APE2
• APEX nuclease (apurinic/apyrimidinic endonuclease) 2
• APEX nuclease 2
• APEXL2
• Apurinic-apyrimidinic endonuclease 2
• DNA-(apurinic or apyrimidinic site) lyase 2
• DNA-apurinic or apyrimidinic site lyase 2
• EC 3.1
• EC 4.2.99.18
• XTH2

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA by spontaneous hydrolysis, DNA damaging agents or DNA glycosylases that remove specific abnormal bases. ClassII AP endonucleases act to repair this damage by cleaving the phosphodiester backbone. It is primarily located in the nucleus but is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APEX2 Antibody (NB100-56625)(2)

Publications using NB100-56625

• Stroik S, Carvajal-Garcia J, Gupta D et Al. Stepwise requirements for polymerases ? and ? in theta-mediated end joining Nature 2023-11-28 [PMID: 37968395]
• Karkhanis V, Wang L, Tae S et al. Protein arginine methyltransferase 7 regulates cellular response to DNA damage by methylating promoter histones H2A and H4 of the polymerase delta catalytic subunit gene, POLD1. J Biol Chem. 2012-08-24 [PMID: 22761421] (WB, Chemotaxis)
  
  Details:
  APEXL2(IMG-466). ChIP: NIH 3T3 cells (Figs 3,5). WB: 3T3-GFP lentiviral infected. knockdown using shRNA (Fig 7). Antibody specificity validated through ChIP assay and western blot knockdown.

Bioinformatics

• Gene Symbol: APEX2
• Entrez:
  • 27301(Human)
• Uniprot:
  • Q9UBZ4(Human)