PRMT7 Antibody

Images

 
Western Blot: PRMT7 Antibody [NBP2-19939] - Sample (30 ug of whole cell lysate) A: PC-3 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: PRMT7 Antibody [NBP2-19939] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Chromatin Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 ug of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected ...read more
Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.
Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 ug of ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PRMT7 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary.
Localization
Cytoplasm; cytosol; Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRMT7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Application Notes
ChIP- Assay dependent
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-19939 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Bovine (83%)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for PRMT7 Antibody

  • [Myelin basic protein]-arginine N-methyltransferase PRMT7
  • EC 2.1.1
  • EC 2.1.1.125
  • EC 2.1.1.126
  • EC 2.1.1.321
  • FLJ10640
  • Histone-arginine N-methyltransferase PRMT7
  • KIAA1933
  • KIAA1933EC 2.1.1.-
  • myelin basic protein-arginine N-methyltransferase
  • PRMT7
  • protein arginine methyltransferase 7
  • protein arginine N-methyltransferase 7

Background

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PRMT7 Antibody (NBP2-19939)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IP, WB.


Filter By Application
IP
(1)
WB
(1)
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Mouse
(1)
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Showing Publications 1 - 3 of 3.
Publications using NBP2-19939 Applications Species
Blanc RS, Vogel G, Chen T et al. PRMT7 Preserves Satellite Cell Regenerative Capacity Cell Rep Feb 16 2016 [PMID: 26854227] (WB, Mouse) WB Mouse
Zhu J, Li X, Cai X et al. Arginine Monomethylation by PRMT7 Controls MAVS-Mediated Antiviral Innate Immunity SSRN Electronic Journal Jan 29 2021
Zhu J, Xiao W A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells STAR protocols Dec 17 2021 [PMID: 34820639] (IP) IP

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRMT7
Uniprot