PRMT7 Antibody

Western Blot: PRMT7 Antibody [NBP2-19939] - Sample (30 ug of whole cell lysate) A: PC-3 7. 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: PRMT7 Antibody [NBP2-19939] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Chromatin Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 ug of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.

Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 ug of PRMT7 antibody 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRMT7 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary.
• Localization: Cytoplasm; cytosol; Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Application Notes: ChIP- Assay dependent
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (83%)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PRMT7 Antibody

• [Myelin basic protein]-arginine N-methyltransferase PRMT7
• EC 2.1.1
• EC 2.1.1.125
• EC 2.1.1.126
• EC 2.1.1.321
• FLJ10640
• Histone-arginine N-methyltransferase PRMT7
• KIAA1933
• KIAA1933EC 2.1.1.-
• myelin basic protein-arginine N-methyltransferase
• PRMT7
• protein arginine methyltransferase 7
• protein arginine N-methyltransferase 7

Background

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for PRMT7 Antibody (NBP2-19939)(3)

Publications using NBP2-19939

• Zhu J, Xiao W A proteomics protocol to identify stimulation-induced binding partners dependent on a specific gene in mammalian cells STAR protocols 2021-12-17 [PMID: 34820639] (IP)
• Zhu J, Li X, Cai X et al. Arginine Monomethylation by PRMT7 Controls MAVS-Mediated Antiviral Innate Immunity Mol Cell 2021-06-25 [PMID: 34171297]
• Blanc RS, Vogel G, Chen T et al. PRMT7 Preserves Satellite Cell Regenerative Capacity Cell Rep 2016-02-16 [PMID: 26854227] (WB, Mouse)

Bioinformatics

• Gene Symbol: PRMT7
• Uniprot:
  • Q9NVM4(Human)