PRMT7 Antibody

Western Blot: PRMT7 Antibody [NBP2-19939] - Sample (30 ug of whole cell lysate) A: PC-3 7. 5% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: PRMT7 Antibody [NBP2-19939] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.

Chromatin Immunoprecipitation: PRMT7 Antibody [NBP2-19939] - NIH3T3 chromatin extract.. A: 5 ug control rabbit IgG. B: 5 ug PRMT7 antibody

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PRMT7 Antibody [NBP2-19939] - Immunohistochemical analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRMT7 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary.
• Localization: Cytoplasm; cytosol; Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRMT7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0), 1.0% BSA and 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for PRMT7 Antibody

• [Myelin basic protein]-arginine N-methyltransferase PRMT7
• EC 2.1.1
• EC 2.1.1.125
• EC 2.1.1.126
• FLJ10640
• Histone-arginine N-methyltransferase PRMT7
• KIAA1933EC 2.1.1.-
• myelin basic protein-arginine N-methyltransferase
• protein arginine methyltransferase 7
• protein arginine N-methyltransferase 7

Background

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT7 Antibody (NBP2-19939)(1)

Publication using NBP2-19939

• Blanc RS, Vogel G, Chen T et al. PRMT7 Preserves Satellite Cell Regenerative Capacity Cell Rep Feb 16 2016 [PMID: 26854227] (WB, Mouse)

Bioinformatics

• Gene Symbol: PRMT7
• Uniprot:
  • Q9NVM4(Human)