Brg1 Antibody

Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.

Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: Brg1 Antibody [NB100-2594] - Whole cell lysate (15 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: Brg1 Antibody [NB100-2594] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit antiBRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: Brg1 Antibody [NB100-2594] - Detection of human BRG1-SMARCA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRG1-SMARCA4 antibody NB100-2594 used for IP at 3 ug per reaction. BRG1-SMARCA4 was also immunoprecipitated by rabbit anti-BRG1-SMARCA4 antibody NBP-22234. For blotting immunoprecipitated BRG1-SMARCA4, NB100-2594 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, ChIP, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Brg1 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human BRG1/SMARCA4 using the numbering given in entry NP_003063.2 (GeneID 6597).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMARCA4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:250-1:2000
  • Immunohistochemistry-Paraffin 1:250 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID 24335282). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  Brg1 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Brg1 Antibody

• ATP-dependent helicase SMARCA4
• BAF190
• BAF190A
• brahma protein-like 1
• Brg1
• BRG1-associated factor 190A
• BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
• BRM/SWI2-related gene 1
• EC 3.6.1
• EC 3.6.4.-
• FLJ39786
• global transcription activator homologous sequence
• homeotic gene regulator
• hSNF2b
• Mitotic growth and transcription activator
• nuclear protein GRB1
• Protein brahma homolog 1
• Protein BRG-1
• RTPS2
• SMARCA4
• SNF2
• SNF2B
• SNF2-beta
• SNF2L4
• SNF2LB
• SNF2-like 4
• subfamily a, member 4
• sucrose nonfermenting-like 4
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI2
• transcription activator BRG1

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Brg1 Antibody (NB100-2594)(4)

Publications using NB100-2594

• Singh AP, Foley JF, Rubino M et al. Brg1 enables rapid growth of the early embryo by suppressing genes that regulate apoptosis and cell growth arrest. Mol. Cell. Biol. May 16 2016 [PMID: 27185875] (ChIP, IP, WB, Mouse)
• Singh AP, Archer TK. Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation. Nucleic Acids Res 2013 Dec 13 [PMID: 24335282] (IP, ChIP, Mouse)
• Miyamoto K, Pasque V, Jullien J et al. Nuclear actin polymerization is required for transcriptional reprogramming of Oct4 by oocytes. Genes Dev 2011 May [PMID: 21536734]
• Goldman JA, Garlick JD, Kingston RE et al. Chromatin remodeling by imitation switch (ISWI) class ATP-dependent remodelers is stimulated by histone variant H2A.Z. J Biol Chem 2010 Feb [PMID: 19940112]

Bioinformatics

• Gene Symbol: SMARCA4
• Entrez:
  • 6597(Human)
  • 20586(Mouse)
• Uniprot:
  • P51532()