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Brg1 Antibody

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Western Blot: Brg1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody NB100-2594 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: Brg1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse ...read more
Immunohistochemistry: Brg1 Antibody [NB100-2594] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using ...read more
Western Blot: Brg1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 on HeLa whole cell lysate using NB100-2594.
Immunohistochemistry: Brg1 Antibody [NB100-2594] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
   

Biological Strategies

      

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Brg1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human BRG1/SMARCA4 using the numbering given in entry NP_003063.2 (GeneID 6597).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMARCA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:250-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 24335282). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Control
Brg1 Lysate (NBP2-65751)
Publications
Read Publications using
NB100-2594 in the following applications:

  • ChIP
    2 publications
  • IP
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Brg1 Antibody

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • Brg1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SMARCA4
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: SMARCA1 Antibody (2H7B8) [NBP2-61879] - Analysis of paraffin-embedded rectum cancer tissues using SMARCA1 mouse mAb with DAB staining.
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Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP1-90015] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.
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Western Blot: SNF5 Antibody [NBP1-90014] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: SNF5 Antibody [NBP1-90014] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SNF5 Antibody [NBP1-90014] - Staining of human tonsil shows nuclear positivity.
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Western Blot: SMARCC1 Antibody [NBP2-20415] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody [NBP2-20415] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCC1 protein stained by SMARCC1 antibody (C2C3), C-term diluted at 1:500. Red-Phalloidin, a cytoskeleton marker- diluted at 1:200. Scale bar = 10 um.
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Western Blot: SNF2H Antibody [NB100-55310] - WB analysis of SNF2H in Mouse Stem Cell lysate. Verified customer review from 1DegreeBio.
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Applications: WB, IP
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Western Blot: ATRX Antibody [NBP1-83077] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ATRX antibody. Remaining relative intensity is presented. Loading control: anti-GAPDH.
Immunohistochemistry-Paraffin: ATRX Antibody [NBP1-83077] - Staining of human liver shows no positivity in hepatocytes as expected.
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Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
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Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
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Immunocytochemistry/Immunofluorescence: ARID1A Antibody [NBP1-88932] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
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Overexpression Lysate
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Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.
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Publications for Brg1 Antibody (NB100-2594)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ChIP, IP, WB.


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Control Lysate(s)

Brg1 Knockout 293T Cell Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMARCA4
Entrez
Uniprot