Brg1 Antibody


Western Blot: BRG1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 on HeLa whole cell lysate using NB100-2594.
Immunohistochemistry-Paraffin: BRG1 Antibody [NB100-2594] - Human breast carcinoma (left) and mouse colon carcinoma CT26. Antibody used at a dilution of 1:500 (0.4ug/ml) and 1:250 (0.8ug/ml).
Immunohistochemistry-Paraffin: BRG1 Antibody [NB100-2594] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:250.
Proximity Ligation Assay: Brg1 Antibody [NB100-2594] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human SMARCA4 (NB100-2594). Image merged from DAPI (2ms) and Texas more
Proximity Ligation Assay: Brg1 Antibody [NB100-2594] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human SMARCA4 (NB100-2594). Image merged from DAPI more

Product Details

Reactivity Hu, Mu, Canine, Equine, Ferret, Primate, Rabbit, Rhesus MacaqueSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP, PLA
0.2 mg/ml

Order Details

Brg1 Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 75 and 125 of human BRG1/SMARCA4 using the numbering given in entry NP_003063.2 (GeneID 6597).
Predicted Species
Equine (100%), Ferret (100%), Primate (100%), Canine (100%), Rhesus Macaque (100%), Rabbit (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
0.2 mg/ml
Immunogen affinity purified


  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunocytochemistry/Immunofluorescence 1:100- 1:2000
  • Immunohistochemistry 1:250-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Proximity Ligation Assay 1:200-1:2000
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 24335282)
Read Publications using
NB100-2594 in the following applications:

  • 2 publications
  • IP
    2 publications
  • WB
    1 publication

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, Duckbill platypus, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, African elephant and Northern white-cheeked gibbon.


Western Blot Image Notes: Detection of Human and Mouse BRG1/SMARCA4 by WB (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody NB100-2594 used for WB at 0.04 mcg/ml (A) and at 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). Immunohistochemistry-Paraffin Image Notes:

Alternate Names for Brg1 Antibody

  • ATP-dependent helicase SMARCA4
  • BAF190
  • BAF190A
  • brahma protein-like 1
  • BRG1-associated factor 190A
  • BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
  • BRM/SWI2-related gene 1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ39786
  • global transcription activator homologous sequence
  • homeotic gene regulator
  • hSNF2b
  • Mitotic growth and transcription activator
  • nuclear protein GRB1
  • Protein brahma homolog 1
  • Protein BRG-1
  • RTPS2
  • SNF2
  • SNF2B
  • SNF2-beta
  • SNF2L4
  • SNF2LB
  • SNF2-like 4
  • subfamily a, member 4
  • sucrose nonfermenting-like 4
  • SWI/SNF related, matrix associated, actin dependent regulator of chromatin
  • SWI2
  • transcription activator BRG1


The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Brg1 Antibody (NB100-2594)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Secondary Antibodies


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Contact Information

Product PDFs


Gene Symbol SMARCA4

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