Western Blot: BRG1 Antibody [NB100-2594] - Detection of Human and Mouse BRG1/SMARCA4 on HeLa whole cell lysate using NB100-2594.
Immunohistochemistry-Paraffin: BRG1 Antibody [NB100-2594] - Human breast carcinoma (left) and mouse colon carcinoma CT26. Antibody used at a dilution of 1:500 (0.4ug/ml) and 1:250 (0.8ug/ml).
Immunohistochemistry-Paraffin: BRG1 Antibody [NB100-2594] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-BRG1/SMARCA4 used at a dilution of 1:250.
Proximity Ligation Assay: Brg1 Antibody [NB100-2594] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human SMARCA4 (NB100-2594). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: Brg1 Antibody [NB100-2594] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human SMARCA4 (NB100-2594). Image merged from DAPI ...read more
Based on 100% sequence identity, this antibody is predicted to react with Panda, Orangutan, Gorilla, Chimpanzee, Duckbill platypus, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, African elephant and Northern white-cheeked gibbon.
Western Blot Image Notes: Detection of Human and Mouse BRG1/SMARCA4 by WB (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-BRG1/SMARCA4 antibody NB100-2594 used for WB at 0.04 mcg/ml (A) and at 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B). Detection: Chemiluminescence with exposure times of 30 seconds (A and B). Immunohistochemistry-Paraffin Image Notes:
Alternate Names for Brg1 Antibody
ATP-dependent helicase SMARCA4
brahma protein-like 1
BRG1-associated factor 190A
BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
BRM/SWI2-related gene 1
global transcription activator homologous sequence
homeotic gene regulator
Mitotic growth and transcription activator
nuclear protein GRB1
Protein brahma homolog 1
subfamily a, member 4
sucrose nonfermenting-like 4
SWI/SNF related, matrix associated, actin dependent regulator of chromatin
transcription activator BRG1
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Alternatively spliced transcripts have been found for this gene, but their full-length natures have not been determined.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.