Apelin Antibody

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282] - Rat lung. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Immunohistochemistry-Paraffin: Apelin Antibody [NBP3-12282] - Rat small intestine. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Apelin Antibody Summary

• Immunogen: Synthetic peptide taken within amino acid region 25-77 on human Apelin protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APLN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:50000
  • Immunohistochemistry 1:50-1:150
  • Immunohistochemistry-Paraffin 1:50-1:150
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Apelin Antibody

• AGTRL1 ligand
• AGTRL1 Lligand
• APEL
• Apelin
• apelin, AGTRL1 ligand
• APJ endogenous ligand
• APLN
• XNPEP2

Background

Apelin is a neuropeptide expressed in the supraoptic and paraventricular nuclei and is an endogenous ligand for APJ, a G protein-coupled orphan receptor which is an alternative coreceptor with CD4 for HIV-1. Apelin and APJ are ubiquitously expressed in peripheral tissues, with highest levels reported for heart and lungs, as well several regions within the central nervous system. The actions of apelin remain largely unknown: Apelin inhibits HIV-1 entry in cells coexpressing CD4 and APJ; the oral intake of Apelin in colostrum and breast milk could have a role in the modulation of the immune responses in neonates; more recent studies have also indicated a role for Apelin in the central control of body fluid homeostasis, by influencing AVP release and drinking behavior. In the cardiovascular system several actions of Apelin have been described, including endothelium-dependent vasodilatation, vasoconstriction through direct action on smooth muscle and positive inotropism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APLN