APAF-1 Antibody (2H9A1) - BSA Free

Images

 
Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)
Immunocytochemistry/ Immunofluorescence: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Flow Cytometry: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis of Hela cells using APAF1 mouse mAb (green) and negative control (red).
Western Blot: APAF-1 Antibody (2H9A1) [NBP2-61868] - Analysis using APAF1 mAb against HEK293 (1) and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: APAF-1 Antibody (2H9A1) [NBP2-61868] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
2H9A1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

APAF-1 Antibody (2H9A1) - BSA Free Summary

Immunogen
Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
APAF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000
Theoretical MW
141.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for APAF-1 Antibody (2H9A1) - BSA Free

  • APAF1
  • APAF-1
  • APAF-1apoptotic protease activating factor 1
  • apoptotic peptidase activating factor 1
  • apoptotic peptidase activating factor
  • apoptotic protease activating factor
  • apoptotic protease-activating factor 1
  • CED4
  • DKFZp781B1145
  • KIAA0413

Background

This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for APAF-1 Antibody (NBP2-61868) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for APAF-1 Antibody (NBP2-61868)

Discover related pathways, diseases and genes to APAF-1 Antibody (NBP2-61868). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for APAF-1 Antibody (NBP2-61868)

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Research Areas for APAF-1 Antibody (NBP2-61868)

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Blogs on APAF-1.

Caspase 9 - an important apoptosis marker
Caspases are essential mediators of programmed cell death and are needed for both the induction of apoptosis as well as for aiding the degradation of cellular structures. Initiator caspases (such as Caspase-9) sense and respond to various signals i...  Read full blog post.

Heat Shock Proteins: An Overview
Heat Shock Proteins (HSPs) are a ubiquitous group of molecular chaperone proteins that have evolved unique mechanisms, within their host cells, to facilitate survival in hostile environments such as heat, oxidative (hypoxia), pH and cold. Under permis...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Bioinformatics

Gene Symbol APAF1