Annexin A3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Annexin A3 Antibody [NBP1-90154] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.

Analysis in human bone marrow and testis tissues using NBP1-90154 antibody. Corresponding ANXA3 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Annexin A3 Antibody [NBP1-90154] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.

Immunohistochemistry-Paraffin: Annexin A3 Antibody [NBP1-90154] - Staining of human testis shows very weak positivity in cells in seminiferous ducts a expected.

Analysis in human cell lines A-431 and HEK293 using Anti-ANXA3 antibody. Corresponding ANXA3 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Annexin A3 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANXA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04 - 0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Annexin A3 Protein (NBP1-90154PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Annexin A3 Antibody - BSA Free

• 35-alpha calcimedin
• Annexin A3
• annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase
• Annexin III
• annexin-3
• ANXA3
• Calcimedin 35-alpha
• calcimedin 35-alpha
• Lipocortin III
• PAP-III
• Placental anticoagulant protein III
• placental anticoagulant protein III, calcimedin 35-alpha

Background

Annexin A3 encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANXA3