ANKRD26 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ANKRD26 Antibody [NBP1-82937] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus & vesicles.

Immunohistochemistry-Paraffin: ANKRD26 Antibody [NBP1-82937] - Staining of human rectum shows strong cytoplasmic positivity with a granular pattern in glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ANKRD26 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: DNAKLKVTVKKQMDKIEELQKNLLNANLSEDEKEQLKKLMELKQSLECNLDQEMKKNVELEREITGFKNL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANKRD26
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ANKRD26 Protein (NBP1-82937PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ANKRD26 Antibody - BSA Free

• ankyrin repeat domain 26
• ankyrin repeat domain-containing protein 26
• bA145E8.1
• KIAA1074MGC163325

Background

The ANKRD26 (ankyrin repeat domain 26) gene has been implicated to give rise to the POTE gene family which encode proteins containing ankyrin repeats and coiled -coil domains. The generation of mice that bear mutant AKFRD26 indicates that ANKRD26 plays a role in obesity, insulin resistance, and body size. ANKRD26 is also known as KIAA1074. Yes-associated protein 1 (YAP1) is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [from NCBI GeneID:10413]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ANKRD26 Antibody (NBP1-82937). (Showing 1 - 1 of 1 FAQ).

1. Is the antibody code. NBP1-82937 suitable for whole blood samples?
   • Our ANKRD26 antibody with catalogue number NBP1-82937 has been validated for IHC with paraffin-embedded human tissue samples, and it has also been validated for immunofluorescence; other species and applications have not yet been tested. The immunocytochemistry application was validated using plated A-431 cells, which are an adherent epidermoid carcinoma line. We do not routinely test our antibodies with samples of whole blood, and unfortunately we have no testing data to confirm whether this particular antibody would work in such an experimental set up. Should you be analysing your whole blood samples by flow cytometry, please note that NBP1-82937 has not yet been tested for flow.

Bioinformatics

• Gene Symbol: ANKRD26