GPS2 Antibody (HL2276)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GPS2 antibody [HL2276] detects GPS2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse salivary gland.GPS2 stained by GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPS2 antibody [HL2276] detects GPS2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat testis.GPS2 stained by GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GPS2 antibody [HL2276] detects GPS2 protein at cytoplasm by immunofluorescent analysis.Sample: 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPS2 stained by GPS2 antibody [HL2276] (NBP3-48675) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Mu, Rt, Ze
• Applications: WB, ICC/IF, IHC
• Clone: HL2276
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

GPS2 Antibody (HL2276) Summary

• Description: Novus Biologicals Rabbit GPS2 Antibody (HL2276) (NBP3-48675) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragmemt of human GPS2.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: GPS2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for GPS2 Antibody (HL2276)

• AMF-1
• G protein pathway suppressor 2
• GPS-2
• MGC104294
• MGC119287
• MGC119288
• MGC119289

Background

GPS2 encodes a protein involved in G protein-mitogen-activated protein kinase (MAPK) signaling cascades. When overexpressed in mammalian cells, this gene could potently suppress a RAS- and MAPK-mediated signal and interfere with JNK activity, suggesting that the function of this gene may be signal repression. The encoded protein is an integral subunit of the NCOR1-HDAC3 (nuclear receptor corepressor 1-histone deacetylase 3) complex, and it was shown that the complex inhibits JNK activation through this subunit and thus could potentially provide an alternative mechanism for hormone-mediated antagonism of AP1 (activator protein 1) function. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GPS2