Angiopoietin-like Protein 4/ANGPTL4 Antibody (547112) [Unconjugated]

• Reactivity: Mu
• Applications: ELISA
• Clone: 547112
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Angiopoietin-like Protein 4/ANGPTL4 Antibody (547112) [Unconjugated] Summary

• Immunogen: CHO-derived mouse Angiopoietin-like Protein 4/ANGPTL4 protein
  Gln24-Ser410
  Accession # Q9Z1P8
• Specificity: Detects mouse Angiopoietin-like Protein 4/ANGPTL4 in direct Elisa.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-like Protein 4/ANGPTL4 Antibody (547112) [Unconjugated]

• Angiopoietin like Protein 4
• angiopoietin-like 4
• Angiopoietin-like Protein 4
• ANGPTL4
• ARP4fasting-induced adipose factor
• FIAF
• FIAFhepatic angiopoietin-related protein
• Hepatic fibrinogen/angiopoietin-related protein
• HFARP
• HFARPANGPTL2
• NL2
• peroxisome proliferator-activated receptor (PPAR) gamma inducedangiopoietin-related protein
• PGAR
• PGARangiopoietin-related protein 4
• pp1158
• PPARG angiopoietin related protein

Background

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature mouse ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with mouse ANGPTL1, 2, 3, 4, 6, and 7. It shares 75% and 97% aa sequence identity with human and rat ANGPTL4, respectively. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins  beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
13. Clement, L.C. et al. (2011) Nat. Med. 17:117.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9Z1P8()