Angiopoietin-2 Antibody (180102) [Unconjugated]

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, HepG2 human hepatocellular carcinoma cell line, human placenta tissue, human ovary tissue, and human uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Angiopoietin‑2 Monoclonal Antibody (Catalog # MAB0983) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Angiopoietin‑2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 180102
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH
• Suitable Sample Type: 23005

Angiopoietin-2 Antibody (180102) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-2
  Asp68-Phe496
  Accession # O15123
• Specificity: Detects human Angiopoietin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, or rmANGPTL3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANGPT2

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-2 Antibody (180102) [Unconjugated]

• AGPT2
• ANG2
• ANG-2
• angiopoietin 2
• Angiopoietin-2
• angiopoietin-2a
• angiopoietin-2B
• angiopoitin 2
• ANGPT2
• Tie2-ligand

Background

Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation (1, 2). Mature Ang-2 is 478 amino acids (aa) in length. It contains one coiled-coil domain (aa 166 - 248) that mediates multimerization, and a C-terminal fibrinogen-like domain (aa 275 - 495) that mediates receptor binding. Under reducing conditions, secreted monomeric Ang-2 is 65 - 66 kDa in size. Under nonreducing conditions, both natural and recombinant Ang-2 form 140 kDa dimers, 200 kDa trimers, and 250 - 300 kDa tetramers and pentamers (3 - 6). Alternate splicing generates a short isoform that lacks 52 amino acids preceding the coiled-coil domain (4). Mature human Ang-2 shares 86% aa sequence identity with mouse and rat Ang-2. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus (3). It is particularly abundant in vascular endothelial cells (EC) where it is stored in intracellular Weibel-Palade bodies (1, 3, 7). Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2 (2). While Ang‑1 is a potent Tie-2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang‑2 becomes as a Tie-2 agonist (3, 8 - 11). The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2 (4). Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression (12, 13). Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells (8, 13, 14). Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development (7, 15). It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation (16). Ang-2 is upregulated in both the endothelium and tumor cells of several cancers as well as in ischemic tissue (17 - 20). Its direct interaction with Integrins promotes tumor cell invasion (21, 22). Ang-2 also promotes the neuronal differentiation and migration of subventricular zone progenitor cells (20).

1. Augustin, H.G. et al. (2009) Nat. Rev. Mol. Cell Biol. 10:165.
2. Murdoch, C. et al. (2007) J. Immunol. 178:7405.
3. Maisonpierre, P.C. et al. (1997) Science 27:55.
4. Kim, I. et al. (2000) J. Biol. Chem. 275:18550.
5. Procopio, W.N. et al. (1999) J. Biol. Chem. 274:30196.
6. Kim, K-T. et al. (2005) J. Biol. Chem. 280:20126.
7. Gale, N.W. et al. (2002) Dev. Cell 3:411.
8. Yuan, H.T. et al. (2009) Mol. Cell. Biol. 29:2011.
9. Falcon, B.L. et al. (2009) Am. J. Pathol. 175:2159.
10. Kim, H-Z. et al. (2009) Biochim. Biophys. Acta 1793:772.
11. Kim, I. et al. (2001) Cardiovasc. Res. 49:872.
12. Lobov, I.B. et al. (2002) Proc. Natl. Acad. Sci. 99:11205.
13. Cao, Y. et al. (2007) Cancer Res. 67:3835.
14. Nasarre, P. et al. (2009) Cancer Res. 69:1324.
15. Dellinger, M. et al. (2008) Dev. Biol. 319:309.
16. Fiedler, U. et al. (2006) Nat. Med. 12:235.
17. Koga, K. et al. (2001) Cancer Res. 61:6248.
18. Etoh, T. et al. (2001) Cancer Res. 61:2145.
19. Tressel, S.L. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1989.
20. Liu, X.S. et al. (2009) J. Biol. Chem. 284:22680.
21. Hu, B. et al. (2006) Cancer Res. 66:775.
22. Imanishi, Y. et al. (2007) Cancer Res. 67:4254.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-2 Antibody (MAB0983)(5)

Publications using MAB0983

• B Kim, C Jang, H Dharaneesw, J Li, M Bhide, S Yang, K Li, Z Arany Endothelial pyruvate kinase M2 maintains vascular integrity J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30222136]
• S Demolli, A Doddaballa, K Devraj, K Stark, Y Manavski, A Eckart, CM Zehendner, T Lucas, T Korff, M Hecker, S Massberg, S Liebner, D Kaluza, RA Boon, S Dimmeler Shear stress-regulated miR-27b controls pericyte recruitment by repressing SEMA6A and SEMA6D Cardiovasc. Res., 2017;113(6):681-691. 2017 [PMID: 28453731] (Western Blot, Human)
• Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN Regulation of angiopoietin expression by bacterial lipopolysaccharide. Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L955-63. 2008 [PMID: 18310225] (Western Blot, Mouse)
• Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia. Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L142-50. 2007 [PMID: 17400600] (IHC-P, Mouse)
• Tseng JJ, Hsu SL, Ho ES, Hsieh YT, Wen MC, Chou MM Differential expression of angiopoietin-1, angiopoietin-2, and Tie receptors in placentas from pregnancies complicated by placenta accreta. Am. J. Obstet. Gynecol., 2006;194(2):564-71. 2006 [PMID: 16458662] (IHC-P, Human)

Bioinformatics

• Gene Symbol: ANGPT2
• Uniprot:
  • O15123()