Recombinant Human Angiopoietin-1 Protein

Product Discontinued

Recombinant Human Angiopoietin-1 Protein Summary

• Description: An un-tagged denatured recombinant protein corresponding to the amino acids 20-498 of Human Angiopoietin-1
  
  

  Source: E.coli

  Amino Acid Sequence: MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNTNALQ RDAPHVEPDF SSQKLQHLEH VMENYTQWLQ KLENYIVENM KSEMAQIQQN AVQNHTATML EIGTSLLSQT AEQTRKLTDV ETQVLNQTSR LEIQLLENSL STYKLEKQLL QQTNEILKIH EKNSLLEHKI LEMEGKHKEE LDTLKEEKEN LQGLVTRQTY IIQELEKQLN RATTNNSVLQ KQQLELMDTV HNLVNLCTKE GVLLKGGKRE EEKPFRDCAD VYQAGFNKSG IYTIYINNMP EPKKVFCNMD VNGGGWTVIQ HREDGSLDFQ RGWKEYKMGF GNPSGEYWLG NEFIFAITSQ RQYMLRIELM DWEGNRAYSQ YDRFHIGNEK QNYRLYLKGH TGTAGKQSSL ILHGADFSTK DADNDNCMCK CALMLTGGWW FDACGPSNLN GMFYTAGQNH GKLNGIKWHY FKGPSYSLRS TTMMIRPLDF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ANGPT1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 55.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 5% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Angiopoietin-1 Protein

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Human ANGPT1 is a secreted ligand for Tie-2, a cell surface receptor tyrosine kinase expressed in endothelial and hemopoietic cells. This protein is glycosylated and has a fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. ANGPT1 is an angiogenic factor that mediates blood vessel maturation and may be involved in endothelial development. Recombinant human ANGPT1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Angiopoietin-1 Recombinant Protein (NBP1-72477). (Showing 1 - 1 of 1 FAQ).

1. We have about 250 microliters of Angiopoietin-1 (NBP1-72477) that we purchased in late 2012. It was accidentally stored at 4 degrees Celsius rather than the suggested -80 degrees Celsius for about six months. We would like to know if you think it would still be viable, or how you would recommend testing for viability?
   • Unfortunately, there is no good way to know the viability of the protein other than simply testing it yourself, as we have never tested our proteins in this manner. I would, however, recommend using more than the recommended amount for your assay as some of the protein will almost certainly have degraded. Most likely there are still functional proteins left in the vial, it will just have to be experimentally determined how much is necessary to obtain optimal results.

Bioinformatics

• Gene Symbol: ANGPT1