Porcine Angiopoietin-1 ELISA Kit (Colorimetric)

Porcine Angiopoietin-1 ELISA Kit (Colorimetric) [NBP2-77020] - Standard Curve Reference

• Reactivity: Po
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and tissue homogenates
• Standard Curve Range: 6 ng/mL - 200 ng/mL
• Sensitivity: 6 ng/mL

Porcine Angiopoietin-1 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of porcine Angiopoietin-1. No significant cross-reactivity or interference between porcine Angiopoietin-1 and analogues was observed.
• Standard Curve Range: 6 ng/mL - 200 ng/mL
• Sensitivity: 6 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Spike Recovery: 90 - 105%
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ANGPT1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Porcine Angiopoietin-1 ELISA Kit (Colorimetric)

• AGP1
• AGPT
• Ang1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Angiopoietin-1 ELISA Kit (NBP2-77020). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ANGPT1