Angiopoietin-1 Antibody [Unconjugated]

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." > CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." title="Angiopoietin-1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." />

Angiopoietin-1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

sAng-2 concentration positively relates with Ang-2 expression on the epithelia and MVD in cervical tissues.Representative immunohistochemical staining of Ang-1 (A), Ang-2 (B) and CD34 (C) in 25 cervical cancer tissue specimens and 10 normal controls. Black arrows denote positively stained epithelial cells, whereas red arrows denote positively staining endothelial cells, all appearing brown. Scale bar, 20 µm. (D) sAng-2 is significantly higher in the patients with positive Ang-2 expression on cervix epithelia than those with negative Ang-2 expression. The scatter diagrams show the correlations of sAng-1 (E), sAng-2 (F) and sAng-1/ sAng-2 ratio (G) to MVD in the 35 cervical tissue specimens. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28584715), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of sst5TMD4 and co-localization with angiogenic marker in GEP-NET(A) Analysis of expression of angiogenic molecules and sst5TMD4 by specific serial immunohistochemistry in a pancreatic NET. Original magnification ×100 and ×400 (insets). N: normal tissue; T: tumor tissue. For specific immunostaining techniques see the “Materials and methods” section. (B) Expression of sst5TMD4 and angiogenic molecules by triple immunofluorescence in a gastrointestinal NET sample. Original magnification ×400. N: normal tissue; T: tumor tissue. For specific immunofluorescence techniques see the “Materials and methods” section. Scale bar for 100 μm is represented with a line for each Figure. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26673010), licensed under a CC-BY license. Not internally tested by R&D Systems.

923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" > 923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" title="Recombinant Human Angiopoietin‑1 (Catalog # 923-AN) was serially diluted and captured by Mouse Anti-Human Angiopoietin‑1 Monoclonal Antibody (Catalog # MAB9231) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" />

Recombinant Human Angiopoietin‑1 (Catalog # <a class=

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Angiopoietin-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
  Ser20-Phe498
  Accession # Q5HYA0
• Specificity: Detects human and mouse Angiopoietin-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ANGPT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-1 Antibody [Unconjugated]

• AGP1
• AGPT
• ANG1
• ANG-1
• angiopoietin 1
• Angiopoietin-1
• ANGPT1
• KIAA0003

Background

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
2. Davis, S. et al. (1996) Cell 87:1161.
3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Angiopoietin-1 Antibody (AF923)(15)

Publications using AF923

• YG Choe, JH Yoon, J Joo, B Kim, SP Hong, GY Koh, DS Lee, WY Oh, Y Jeong Pericyte Loss Leads to Capillary Stalling Through Increased Leukocyte-Endothelial Cell Interaction in the Brain Frontiers in Cellular Neuroscience, 2022-03-11;16(0):848764. 2022-03-11 [PMID: 35360491] (Western Blot, Mouse)
• G Wang, MR de Vries, WMPJ Sol, AM van Oevere, HC de Boer, AJ van Zonnev, PHA Quax, TJ Rabelink, BM van den Be Loss of Endothelial Glycocalyx Hyaluronan Impairs Endothelial Stability and Adaptive Vascular Remodeling After Arterial Ischemia Cells, 2020-03-29;9(4):. 2020-03-29 [PMID: 32235347] (IHC, Mouse)
• B Okyere, WA Mills Iii, X Wang, M Chen, J Chen, A Hazy, Y Qian, JB Matson, MH Theus EphA4/Tie2 crosstalk regulates leptomeningeal collateral remodeling following ischemic stroke J. Clin. Invest., 2020-02-03;0(0):. 2020-02-03 [PMID: 31689239] (Western Blot, Mouse)
• Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini Impact of heat therapy on recovery after eccentric exercise in humans Journal of Applied Physiology 4/1/2019 [PMID: 30605396]
• Zheng C, Toth J, Bigwarfe T et al. Non-neutralizing antibodies increase endogenous circulating Ang1 levels MAbs 9/11/2018 [PMID: 30199300]
• D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury Front Pharmacol, 2018-04-27;9(0):369. 2018-04-27 [PMID: 29755348] (Western Blot, Mouse)
• Swati Shrestha Ang-2 but not Ang-1 expression in perivascular soft tissue tumors J Orthop, 2016-11-28;14(1):147-153. 2016-11-28 [PMID: 27942190] (IHC-P, Human)
• Sampedro-Nunez M, Luque RM, Ramos-Levi AM et al. Presence of sst5TMD4, a truncated splice variant of the somatostatin receptor subtype 5, is associated to features of increased aggressiveness in pancreatic neuroendocrine tumors. Oncotarget [PMID: 26673010]
  
  Details:
  Used the Alexa Fluor 488 form of this antibody.
• Baruah P, Lee M, Wilson P, Odutoye T, Williamson P, Hyde N, Kaski J, Dumitriu I Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers. Br J Cancer, 2015-07-14;113(4):653-9. 2015-07-14 [PMID: 26171937] (IHC-P, Human)
• Joseph M. McClung, Jessica L. Reinardy, Sarah B. Mueller, Timothy J. McCord, Christopher D. Kontos, David A. Brown et al. Muscle cell derived angiopoietin-1 contributes to both myogenesis and angiogenesis in the ischemic environment Frontiers in Physiology 5/19/2015 [PMID: 26042050]
• Brunckhorst MK, Wang H, Lu R, Yu Q Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res., 2010-09-07;70(18):7283-93. 2010-09-07 [PMID: 20823154] (Western Blot, Human)
• Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy. FASEB J., 2008-05-23;22(8):3010-23. 2008-05-23 [PMID: 18502941] (Western Blot, Mouse)
• Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction. Clin. Immunol., 2007-06-08;124(2):158-64. 2007-06-08 [PMID: 17560831] (ELISA Development, Human)
• Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-05-05;291(4):L588-95. 2006-05-05 [PMID: 16679381] (Western Blot, Primate - Papio anubis (Olive Baboon))
• Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Proc. Natl. Acad. Sci. U.S.A., 2005-07-11;102(29):10212-7. 2005-07-11 [PMID: 16009933] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ANGPT1
• Uniprot:
  • Q5HYA0()