Angiopoietin-1 Antibody [Unconjugated]

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Angiopoietin‑1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL ...read more
Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Angiopoietin‑1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Angiopoietin-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Angiopoietin-1
Ser20-Phe498
Accession # Q5HYA0
Specificity
Detects human and mouse Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Angiopoietin-2 and rhAngiopoietin-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ANGPT1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF923 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Angiopoietin-1 Antibody [Unconjugated]

  • AGP1
  • AGPT
  • Ang1
  • ANG-1
  • angiopoietin 1
  • Angiopoietin-1
  • ANGPT1
  • KIAA0003

Background

Angiopoietin-1 (Ang-1) and Angiopoietin-2 (Ang-2) are two closely related secreted ligands which bind with similar affinity to Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells. Tie-2 and angiopoietins have been shown to play critical roles in embryogenic angiogenesis and in maintaining the integrity of the adult vasculature (1).

Ang-1 cDNA encodes a 498 amino acid (aa) residue precursor protein that contains a coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Human Ang-1 shares approximately 97% and 60% amino acid sequence identity with mouse Ang-1 and human Ang-2, respectively (1, 2). Ang-1 activates Tie-2 signaling on endothelial cells to promote chemotaxis, cell survival, cell sprouting, vessel growth and stabilization (1, 3, 4). Ang-2 has alternatively been reported to be an antagonist for Ang-1 induced Tie-2 signaling as well as an agonist for Tie-2 signaling, depending on the cell context (5).

  1. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  2. Davis, S. et al. (1996) Cell 87:1161.
  3. Witzenbichler, B. et al. (1998) J. Biol. Chem. 273:18514.
  4. Papapetropoulos, A. et al. (1999) Lab. Inest. 79:213.
  5. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Angiopoietin-1 Antibody (AF923)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Papio anubis (Olive Baboon).

We have publications tested in 4 applications: ELISA Development, IHC, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
IHC
(1)
IHC-P
(2)
Western Blot
(6)
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Filter By Species
Human
(5)
Mouse
(4)
Primate - Papio anubis (Olive Baboon)
(1)
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Showing Publications 1 - 10 of 10.
Publications using AF923 Applications Species
G Wang, MR de Vries, WMPJ Sol, AM van Oevere, HC de Boer, AJ van Zonnev, PHA Quax, TJ Rabelink, BM van den Be Loss of Endothelial Glycocalyx Hyaluronan Impairs Endothelial Stability and Adaptive Vascular Remodeling After Arterial Ischemia Cells, 2020;9(4):. 2020 [PMID: 32235347] (IHC, Mouse) IHC Mouse
B Okyere, WA Mills Iii, X Wang, M Chen, J Chen, A Hazy, Y Qian, JB Matson, MH Theus EphA4/Tie2 crosstalk regulates leptomeningeal collateral remodeling following ischemic stroke J. Clin. Invest., 2019;0(0):. 2019 [PMID: 31689239] (Western Blot, Mouse) Western Blot Mouse
D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury Front Pharmacol, 2018;9(0):369. 2018 [PMID: 29755348] (Western Blot, Mouse) Western Blot Mouse
Swati Shrestha Ang-2 but not Ang-1 expression in perivascular soft tissue tumors J Orthop, 2017;14(1):147-153. 2017 [PMID: 27942190] (IHC-P, Human) IHC-P Human
Baruah P, Lee M, Wilson P, Odutoye T, Williamson P, Hyde N, Kaski J, Dumitriu I Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers. Br J Cancer, 2015;113(4):653-9. 2015 [PMID: 26171937] (IHC-P, Human) IHC-P Human
Brunckhorst MK, Wang H, Lu R, Yu Q Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis. Cancer Res., 2010;70(18):7283-93. 2010 [PMID: 20823154] (Western Blot, Human) Western Blot Human
Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy. FASEB J., 2008;22(8):3010-23. 2008 [PMID: 18502941] (Western Blot, Mouse) Western Blot Mouse
Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Menard JF, Pouplin S, Boumier P, Vittecoq O, Le Loet X, Boissier MC Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction. Clin. Immunol., 2007;124(2):158-64. 2007 [PMID: 17560831] (ELISA Development, Human) ELISA Development Human
Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo. Am. J. Physiol. Lung Cell Mol. Physiol., 2006;291(4):L588-95. 2006 [PMID: 16679381] (Western Blot, Primate - Papio anubis (Olive Baboon)) Western Blot Primate - Papio anubis (Olive Baboon)
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Gunzler V, White CW Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung. Proc. Natl. Acad. Sci. U.S.A., 2005;102(29):10212-7. 2005 [PMID: 16009933] (Western Blot, Human) Western Blot Human

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Secondary Antibodies

 

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Biotin BAF923

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Bioinformatics

Gene Symbol ANGPT1
Uniprot