Androgen R/NR3C4 Antibody (523339) [Phycoerythrin]

LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 PE-conjugated Monoclonal Antibody (Catalog # IC5876P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu, Mu, Rt
• Applications: Flow
• Clone: 523339
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Androgen R/NR3C4 Antibody (523339) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human Androgen R/NR3C4
  Thr660-Gln919
  Accession # P10275
• Specificity: Detects human Androgen R/NR3C4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: AR
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Androgen R/NR3C4 Antibody (523339) [Phycoerythrin]

• AIS
• Androgen R
• androgen receptor
• AndrogenR
• AR
• DHTR
• DHTRTFM
• Dihydrotestosterone receptorHYSP1
• HUMARA
• NR3C4
• NR3C4KD
• Nuclear receptor subfamily 3 group C member 4
• SBMA
• SMAX1
• SMAX1SBMA
• spinal and bulbar muscular atrophy

Background

AR (Androgen Receptor), also known as NR3C4, is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, macrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR does homodimerize, apparently with multiple isotypes. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Androgen R/NR3C4 Antibody (IC5876P)(1)

Publication using IC5876P

• M Pizon, D Lux, U Pachmann, K Pachmann, D Schott Influence of endocrine therapy on the ratio of androgen receptor (AR) to estrogen receptor (ER) positive circulating epithelial tumor cells (CETCs) in breast cancer J Transl Med, 2018-12-14;16(1):356. 2018-12-14 [PMID: 30547831] (ICC, Human)

Bioinformatics

• Gene Symbol: AR
• Uniprot:
  • P10275()