AMPK beta 1 Antibody [PE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: PE

AMPK beta 1 Antibody [PE] Summary

• Immunogen: E. coli-derived recombinant human AMPK beta 1
  Met1-Ile270
  Accession # Q9Y478
• Specificity: Detects human, mouse, and rat AMPK beta 1 in Western blots. In Western blots, no cross-reactivity with recombinant human AMPK beta 2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRKAB1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for AMPK beta 1 Antibody [PE]

• 5'-AMP-activated protein kinase beta-1 subunit
• AMP-activated protein kinase beta subunit
• AMPK beta -1 chain
• AMPK beta 1
• AMPK beta 1,5'-AMP-activated protein kinase subunit beta-1
• AMPK subunit beta-1
• AMPK
• AMPKb
• HAMPKb
• MGC17785
• PRKAB1
• protein kinase, AMP-activated, beta 1 non-catalytic subunit
• protein kinase, AMP-activated, noncatalytic, beta-1

Background

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1). AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAB1