AMPK alpha 2 Antibody (847123) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-468 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human AMPKa2 Monoclonal Antibody (Catalog # MAB2850) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPKa2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 71 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK alpha 2 Monoclonal Antibody (Catalog # MAB2850) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

AMPKa2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human AMPKa2 Monoclonal Antibody (Catalog # MAB2850) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clone: 847123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

AMPK alpha 2 Antibody (847123) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human AMPK alpha 2
  Phe340-Arg552
  Accession # P54646
• Specificity: Detects human AMPK alpha 2 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AMPK alpha 1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKAA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK alpha 2 Antibody (847123) [Unconjugated]

• 5'-AMP-activated protein kinase catalytic subunit alpha-2
• AMPK alpha 2
• AMPK subunit alpha-2
• AMPK2
• AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
• AMPK-alpha-2 chain
• EC 2.7.11
• EC 2.7.11.1
• PRKAA
• PRKAA2
• protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and δ subunits. The alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. Active AMPK down-regulates anabolic pathways such as fatty acid and cholesterol synthesis, and up-regulates catabolic pathways such as glycolysis and fatty acid oxidation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPK alpha 2 Antibody (MAB2850)(3)

Publications using MAB2850

• Benjamin D. Stein, Sébastien Herzig, Salvador Martínez-Bartolomé, Mathieu Lavallée-Adam, Reuben J. Shaw, John R. Yates Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPK alpha 2 Interactome Journal of Proteome Research 2019-10-04 [PMID: 31398040] (Immunoprecipitation, Human)
• Benjamin D. Stein, Sébastien Herzig, Salvador Martínez-Bartolomé, Mathieu Lavallée-Adam, Reuben J. Shaw, John R. Yates Comparison of CRISPR Genomic Tagging for Affinity Purification and Endogenous Immunoprecipitation Coupled with Quantitative Mass Spectrometry To Identify the Dynamic AMPK alpha 2 Interactome Journal of Proteome Research 2019-10-04 [PMID: 31398040]
• Dai S, Dulcey AE, Hu X et al. Methyl-B-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017-06-14 [PMID: 28613987]

Bioinformatics

• Gene Symbol: PRKAA2
• Uniprot:
  • P54646()