Home » AMPK alpha 1 » AMPK alpha 1 Antibodies » AMPK alpha 1 Antibody [Unconjugated]

AMPK alpha 1 Antibody [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for AMPK alpha 1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Goat ...read more
AMPK alpha 1 was detected in immersion fixed paraffin-embedded sections of human liver using 5 µg/mL Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3197) overnight ...read more
AMPK alpha 1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human/Mouse/Rat AMPK alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3197) at 5 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF3197-SP
100% Guarantee on every product

AMPK alpha 1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human AMPK alpha 1
Lys349-Gln559
Accession # Q13131
Specificity
Detects human, mouse, and rat AMPK alpha 1 in Western blots. The antibody does not cross-react with recombinant human AMPK alpha 2.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PRKAA1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3197 in the following applications:
Publications
Read Publications using
AF3197 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK alpha 1 Antibody [Unconjugated]

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic  alpha subunit and regulatory beta  and gamma  subunits. Each subunit exists as alternate isoforms ( alpha 1,  alpha 2, beta 1,  beta 2,  gamma 1,  gamma 2,  gamma 3), with all 12 combinations able to form complexes. The catalytic alpha  subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinases LKB1 and CaMKK beta . AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB
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Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody NB100-1595 (lot NB100-1595-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1595-1). For blotting immunoprecipitated RPS6, NB100-1595 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Analysis of Fatty Acid Synthase, using NB400-114. Samples: 50 ug of total mouse liver lysate.
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(SC06-15)
NBP2-67627
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.
(1 Review)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AMPK alpha 1
Antibody [Unconjugate
AF3197
AMPK alpha 1 Antibody [Unconjugated]
Species: Hu, Mu, Rt
Applications: WB, IHC, ICC/IF

Publications for AMPK alpha 1 Antibody (AF3197)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IHC, Immunoprecipitation, Western Blot, Western Blot`.


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IHC
(1)
Immunoprecipitation
(1)
Western Blot
(2)
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(1)
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Showing Publications 1 - 5 of 5.
Publications using AF3197 Applications Species
W Yang, HY Shin, H Cho, JY Chung, EJ Lee, JH Kim, ES Kang TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS Levels Cancers (Basel), 2020;12(5):. 2020 [PMID: 32456076] (Western Blot`, Human) Western Blot` Human
CH Shi, C Rubel, SE Soss, R Sanchez-Ho, S Zhang, SC Madrigal, S Ravi, H McDonough, RC Page, WJ Chazin, C Patterson, CY Mao, MS Willis, HY Luo, YS Li, DA Stevens, MB Tang, P Du, YH Wang, ZW Hu, YM Xu, JC Schisler Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16 PLoS Genet., 2018;14(9):e1007664. 2018 [PMID: 30222779] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
ODK Maddocks, D Athineos, EC Cheung, P Lee, T Zhang, NJF van den Br, GM Mackay, CF Labuschagn, D Gay, F Kruiswijk, J Blagih, DF Vincent, KJ Campbell, F Ceteci, OJ Sansom, K Blyth, KH Vousden Modulating the therapeutic response of tumours to dietary serine and glycine starvation Nature, 2017;544(7650):372-376. 2017 [PMID: 28425994] (Western Blot, Mouse) Western Blot Mouse
Manon Domise AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo Sci Rep, 2016;6(0):26758. 2016 [PMID: 27230293] (IHC, Mouse) IHC Mouse
Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons. J. Biol. Chem., 2012;287(24):19985-96. 2012 [PMID: 22528490] (Western Blot, Mouse) Western Blot Mouse

Review for AMPK alpha 1 Antibody (AF3197) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3197:
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Images Ratings Applications Species Date Details
Western Blot AMPK alpha 1 AF3197
Enlarge 		5
reviewed by:
Verified Customer
 WB Mouse 10/11/2019
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Summary

ApplicationWestern Blot
Sample TestedEndothelial
SpeciesMouse
Lotwmp041909a

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Secondary Antibodies

 

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AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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SH-SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/RatEphrin-B2 Antigen Affinity-purified Polyclonal Antibody (Catalog #AF496,filled histogram) or Normal Goat IgG Isotype Control Antibody(Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugatedAnti-Goat IgG Secondary Antibody (Catalog #F0107).
Goat IgG Isotype Control [Unconjugated]
AB-108-C
IL-6 [HRP]
IL-6 [HRP]
D6050

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Western Blot AMPK alpha 1 AF3197
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 Verified Customer
10/11/2019
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol PRKAA1
Uniprot