Human Amphiregulin ELISA Kit (Colorimetric) Summary
| Sensitivity |
10 pg/mL |
| Assay Type |
Competitive ELISA |
| Inter-Assay |
CV% < 12% |
| Intra-Assay |
CV% < 10% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
AREG |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
AREG (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human AREG. Use in ELISA reported in scientific literature (PMID 24860833) |
| Publications |
|
Reactivity Notes
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- AR Microplate (Item A): coated with anti-human AR.
- Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative.For Standard/Sample (serum/plasma) diluent.
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample (cellculture medium/urine) diluent.
- Detection Antibody AR (Item F): biotinylated anti-human AR (each vial isenough to assay half microplate)
- Standards (Item C) Recombinant human AR.
- Stop Solution (Item I): 0.2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 3, 3’, 5, 5’- tetramethylbenzidine(TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Amphiregulin ELISA Kit (Colorimetric)
Background
The protein encoded by the Amphiregulin gene is a member of the epidermal growth factor family. It is an autocrine growth factor aswell as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) andtransforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote thegrowth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encodedprotein is associated with a psoriasis-like skin phenotype. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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