Amphiregulin Antibody

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Recombinant Human Amphiregulin (Catalog # 262‑AR) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Amphiregulin Antibody Summary

Immunogen
E. coli-derived recombinant human Amphiregulin
Val107-Lys184
Accession # P15514
Specificity
Detects human Amphiregulin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) EGF, rhTGF-alpha, and rhHB-EGF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AREG
Purity
Protein A or G purified
Purity Statement
Protein A or G purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 5-20 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Amphiregulin Antibody

  • Amphiregulin
  • AR
  • AREG
  • AREGB
  • Colorectum cell-derived growth factor
  • CRDGF
  • MGC13647
  • schwannoma-derived growth factor
  • SDGF

Background

Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5‑10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol AREG
Uniprot