Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

Western Blot: Aminopeptidase P1/XPNPEP1 Antibody [NBP2-98604] - Anti-Aminopeptidase P1/XPNPEP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 72 kDa (We are unsure as to the identity of these extra bands).

Immunocytochemistry/Immunofluorescence: Aminopeptidase P1/XPNPEP1 Antibody [NBP2-98604] - Immunofluorescence staining of Aminopeptidase P1/XPNPEP1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98604 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Aminopeptidase P1/XPNPEP1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Aminopeptidase P1/XPNPEP1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: XPNPEP1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Aminopeptidase P1/XPNPEP1 Antibody - BSA Free

• Aminoacylproline aminopeptidase
• Aminopeptidase P1
• APP1
• Cytosolic aminopeptidase P
• cytosolic
• EC 3.4.11.9
• SAMP
• xaa-Pro aminopeptidase 1
• XPNPEP1
• XPNPEPLX-prolyl aminopeptidase (aminopeptidase P)-like
• X-prolyl aminopeptidase (aminopeptidase P) 1, soluble
• X-prolyl aminopeptidase 1, soluble

Background

XPNPEP1 is a proline-specific metalloaminopeptidase that specifically catalyzes the removal of any unsubstituted N-terminal amino acid that is adjacent to a penultimate proline residue. As a result of its specificity toward proline, it has been suggested that XPNPEP1 is important in the maturation and degradation of peptide hormones, neuropeptides, and tachykinins, as well as in the digestion of otherwise resistant dietary protein fragments, thereby complementing the pancreatic peptidases. Deficiency of XPNPEP1 results in excretion of large amounts of imino-oligopeptides in urine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: XPNPEP1