Home » Aminopeptidase N/CD13 » Aminopeptidase N/CD13 Antibodies » Aminopeptidase N/CD13 Antibody (ER-BMDM1)

Aminopeptidase N/CD13 Antibody (ER-BMDM1)

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Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.
Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Analysis of an FFPE tissue section of the mouse lymph node using 1:200 dilution of CD13 antibody.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of mouse peripheral blood granulocytes with rat anti-mouse CD13.
Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Mouse lymph node cryosection anti-CD13 antibody.
Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of a mouse tail cryosection with anti-mouse CD13 antibody.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P
Clone
ER-BMDM1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-64843AF405
Alexa Fluor 488
NB100-64843AF488
Alexa Fluor 647
NB100-64843AF647
Alexa Fluor 700
NB100-64843AF700
Allophycocyanin
NB100-64843APC
DyLight 350
NB100-64843UV
DyLight 405
NB100-64843V
DyLight 488
NB100-64843G
DyLight 550
NB100-64843R
DyLight 594
NB100-64843DL594
DyLight 650
NB100-64843C
DyLight 680
NB100-64843FR
DyLight 755
NB100-64843IR
PE
NB100-64843PE
PerCP
NB100-64843PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-80574
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Aminopeptidase N/CD13 Antibody (ER-BMDM1) Summary

Immunogen
Aminopeptidase N/CD13 Antibody (ER-BMDM1) made from Balb/c bone marrow derived macrophages.
Localization
Cell membrane; single-pass type II membrane protein
Specificity
NB100-64843 recognizes the murine homologue of human CD13.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ANPEP
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:50
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
For Flow Cytometry: Use 10 uL of the suggested working dilution to label 10^6 cells in 100 uL.
Publications
Read Publications using
NB100-64843 in the following applications:

  • Flow
    2 publications
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Aminopeptidase N/CD13 Antibody (ER-BMDM1)

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

Human CD13 is expressed on the majority of peripheral blood monocytes and granulocytes. It is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses (HCV).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Aminopeptidase
N/CD13 Antibody (ER-B ...
NB100-64843
Aminopeptidase N/CD13 Antibody (ER-BMDM1)
Species: Mu
Applications: Flow, IHC, IHC-Fr, IHC-P

Publications for Aminopeptidase N/CD13 Antibody (NB100-64843)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Flow, IHC.


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NB100-64843AF405
Alexa Fluor 488 NB100-64843AF488
Alexa Fluor 647 NB100-64843AF647
Alexa Fluor 700 NB100-64843AF700
Allophycocyanin NB100-64843APC
DyLight 350 NB100-64843UV
DyLight 405 NB100-64843V
DyLight 488 NB100-64843G
DyLight 550 NB100-64843R
DyLight 594 NB100-64843DL594
DyLight 650 NB100-64843C
DyLight 680 NB100-64843FR
DyLight 755 NB100-64843IR
PE NB100-64843PE
PerCP NB100-64843PCP
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Gene Symbol ANPEP
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