Aminopeptidase N/CD13 Antibody (ER-BMDM1)

Images

 
Immunohistochemistry-Paraffin: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody.
Flow Cytometry: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13 (NB100-64843).
Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Mouse lymph node cryosection with Rat anti Mouse CD13 antibody,
Immunohistochemistry-Frozen: Aminopeptidase N/CD13 Antibody (ER-BMDM1) [NB100-64843] - Mouse lymph node cryosection with Rat anti Mouse CD13 antibody.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, IHC-P
Clone
ER-BMDM1
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Aminopeptidase N/CD13 Antibody (ER-BMDM1) Summary

Immunogen
Balb/c bone marrow derived macrophages.
Localization
Cell membrane; single-pass type II membrane protein
Specificity
NB100-64843 recognizes the murine homologue of human CD13.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
ANPEP
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Aminopeptidase N/CD13 Antibody (ER-BMDM1)

  • alanyl (membrane) aminopeptidase
  • Alanyl aminopeptidase
  • Aminopeptidase M
  • Aminopeptidase N
  • ANPEP
  • AP-M
  • APN
  • AP-N
  • CD13 antigen
  • CD13
  • CD13APN
  • EC 3.4.11
  • EC 3.4.11.2
  • gp150
  • LAP1
  • Microsomal aminopeptidase
  • Myeloid plasma membrane glycoprotein CD13
  • p150
  • PEPN
  • PEPNhAPN

Background

Human CD13 is expressed on the majority of peripheral blood monocytes and granulocytes. It is also expressed by the majority of acute myeloid leukemias, chronic myeloid leukemias in myeloid blast crisis, a smaller percentage of lymphoid leukemias and myeloid cell lines. CD13 is also found in several types of non hematopoietic cells such as fibroblasts and endothelial cells and in a soluble form in blood plasma. CD13 is not expressed on B cells, T cells, platelets or erythrocytes. In the mouse, CD13 is a non-covalently linked homodimer of approximately 150kD subunits expressed by a variety of cells including monocytes, macrophages, dendritic cell and veiled cells. CD13 plays a role in biologically active peptide metabolism, in the control of growth and differentiation, in phagocytosis and in bactericidal/tumoricidal activities. CD13 also serves as a receptor for human coronaviruses (HCV).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-64843AF405
Alexa Fluor 488 Labeled NB100-64843AF488
Alexa Fluor 647 Labeled NB100-64843AF647
Alexa Fluor 700 Labeled NB100-64843AF700
Allophycocyanin Labeled NB100-64843APC
DyLight 350 Labeled NB100-64843UV
DyLight 405 Labeled NB100-64843V
DyLight 488 Labeled NB100-64843G
DyLight 680 Labeled NB100-64843FR
DyLight 755 Labeled NB100-64843IR
PE Labeled NB100-64843PE
PerCP Labeled NB100-64843PCP

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Bioinformatics

Gene Symbol ANPEP
Entrez
Uniprot