Aminopeptidase N/CD13 Antibody (22A5) - Azide and BSA Free

Flow Cytometry: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Analysis using the FITC conjugate of NBP1-28429. Staining of granulocytes.

CyTOF-reported: Aminopeptidase N/CD13 Antibody (22A5) [NBP1-28429] - Human bone marrow underwent RBC lysis and surface staining with Gd-160 conjugate of CD13 (22A5) antibody at 2 ug/mL, followed by gating on standard surface markers. Neutrophils demonstrate characteristic CD13 loss and reacquisition, with partial reactivity on monocytes. Specificity is very good, with minimal reactivity on lymphocytes. Image from verified customer review.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 22A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.1 mg/ml

Aminopeptidase N/CD13 Antibody (22A5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Aminopeptidase N/CD13 Antibody (22A5) - Azide and BSA Free (NBP1-28429) is a monoclonal antibody validated for use in Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Osteoclasts from osteoclastomas
• Specificity: Human CD13
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ANPEP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry <= 1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Borate buffered saline, pH 8.2
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Aminopeptidase N/CD13 Antibody (22A5) - Azide and BSA Free

• alanyl (membrane) aminopeptidase
• Alanyl aminopeptidase
• Aminopeptidase M
• Aminopeptidase N
• ANPEP
• AP-M
• APN
• AP-N
• CD13 antigen
• CD13
• CD13APN
• EC 3.4.11
• EC 3.4.11.2
• gp150
• LAP1
• Microsomal aminopeptidase
• Myeloid plasma membrane glycoprotein CD13
• p150
• PEPN
• PEPNhAPN

Background

CD13, also known as aminopeptidase N, is a 150 kDa type II integral membrane glycoprotein. It is expressed on granulocytes, monocytes and their precursors, and on mast cells. CD13 is a zinc-binding metalloprotease that is involved in cell surface antigen presentation via its digestion of many MHC-bound regulatory peptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Aminopeptidase N/CD13 Antibody (NBP1-28429) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Albert Tsai CyTOF Human 11/14/2018

Summary

• Application: CyTOF
• Sample Tested: Bone marrow cells
• Species: Human
• Lot: N/A

Comments

• Comments: Human bone marrow underwent RBC lysis and surface staining with Gd 160 CD13 22A5 at 2 ug/mL, followed by gating on standard surface markers. Neutrophils demonstrate characteristic CD13 loss and reacquisition, with partial reactivity on monocytes. Specificity is very good, with minimal reactivity on lymphocytes.
  
  I threw away tube so I don't have the lot number, ordered through Fisher Scientific on August 17, 2017 for Albert Tsai of the Bendall Lab at Stanford University Req 4308201 PO 61584528

FAQs for Aminopeptidase N/CD13 Antibody (NBP1-28429). (Showing 1 - 1 of 1 FAQ).

1. I would like to know the expiry date of the following products: NBP1-47232, NB110-87060, and NBP1-28429
   • We have a 6 month guarantee of our antibodies from the date they are received by your lab. This does not necessarily go off the lot number. The antibodies often can work longer than this date if stored properly, but they cannot be guaranteed.

Bioinformatics

• Gene Symbol: ANPEP