alpha Tubulin 4a Antibody (2E11) - Azide and BSA Free

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - TUBA1 monoclonal antibody (M01), clone 2E11. Analysis of TUBA1 expression in different Cell lines and Human Tissue.

Immunocytochemistry/Immunofluorescence: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of monoclonal antibody to TUBA1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi ( Cat # H00007277-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody (M01), clone 2E11 (Cat # H00007277-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - TUBA1 monoclonal antibody (M01), clone 2E11. Analysis of TUBA1 expression in HeLa.

Western Blot: alpha Tubulin 4a Antibody (2E11) [H00007277-M01] - Analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody (M01), clone 2E11.Lane 1: TUBA1 transfected lysate(49.9 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, Mycoplasma
• Clone: 2E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

alpha Tubulin 4a Antibody (2E11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TUBA1 (AAH09238, 1 a.a. ~ 448 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRECISVHVGQAGVQMGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFTTFFCETGAGKHVPRAVFVDLEPTVIDEIRNGPYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDPVLDRIRKLSDQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIAAIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGIDSYEDEDEGEE
• Specificity: TUBA1 - tubulin, alpha 1 (testis specific)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TUBA4A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for alpha Tubulin 4a Antibody (2E11) - Azide and BSA Free

• alpha 1 (testis specific)
• alpha Tubulin 4a
• Alpha-tubulin 1
• FLJ30169
• H2-ALPHA
• Testis-specific alpha-tubulin
• TUBA1
• TUBA4A
• Tubulin alpha-1 chain
• tubulin alpha-4A chain
• Tubulin H2-alpha
• tubulin, alpha 1
• tubulin, alpha 4a

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TUBA4A
• Entrez:
  • 7277(Human)
• OMIM:
  • 191110(Human)
• Uniprot:
  • AAH09238(Human)