Synthetic peptide corresponding to N-terminus of human alpha smooth muscle isoform of actin.
Mesenchymal Cell Marker
This alpha-sma antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25783396). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted by homology: bovine, chicken, frog, porcine, and rabbit, mouse, rat.
Alternate Names for alpha-Smooth Muscle Actin Antibody
Actin alpha 2
actin, alpha 2, smooth muscle, aorta
actin, aortic smooth muscle
alpha 2 actin
alpha smooth muscle actin
alphaSmooth Muscle Actin
Cell growth-inhibiting gene 46 protein
growth-inhibiting gene 46
smooth muscle actin
Actins are highly conserved proteins expressed in all eukaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for alpha-Smooth Muscle Actin Antibody (NB600-531). (Showing 1 - 1 of 1 FAQs).
I'd like to use this antibody on paraffin embedded sections. What kind of antigen retrieval should I use?
We did not use a customized protocol for the validation of this product and our general IHC-P protocol may be found here. The antigen retrieval method which is routinely employed in our lab is Citrate buffer based Heat Induced Epitope Retrieval (HIER) which as follows: Dissolve 1.92 g of Citric acid (anhydrous) in 1000 ml of distilled water. Adjust pH to 6.0 w/ 1N NaOH and add 0.5 ml of Tween 20, mix well. Pre-heat steamer or water bath with staining dish containing Sodium Citrate Buffer or Citrate Buffer until temperature reaches 95-100 degrees Celcius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure. NB600-531 is one of our best sellers with nice customer feedback and citations in at least 13 peer reviewed publications in journals of high repute. In fact in many publications the authors have used this product for IHC application and you may follow the same to see what would work best for your samples and to compare your outcome. Please keep us posted with your results from the assay and kindly do not hesitate to contact us again if additional feedback is required or if you have other queries on any of our products.
Understanding Actin Alpha 2 Smooth Muscle Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated... Read full blog post.