alpha-Smooth Muscle Actin Antibody


Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NB600-531] - Formalin-fixed, paraffin-embedded human Leiomyoma stained with alpha-sma antibody.
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody [NB600-531] - Human small intestine stained with anti-alpha-smooth muscle Actin antibody.

Product Details

Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

alpha-Smooth Muscle Actin Antibody Summary

Synthetic peptide corresponding to N-terminus of human alpha smooth muscle isoform of actin.
Mesenchymal Cell Marker
This alpha-sma antibody stains smooth muscle cells in vessel walls, gut wall, and myometrium. Myoepithelial cells in breast and salivary gland are also stained. It reacts with tumors arising from smooth muscles and myoepithelial cells.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS/1% BSA buffer pH 7.6
0.1% Sodium Azide
1.0 mg/ml
Immunogen affinity purified


Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25783396). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
42 kDa
Read Publications using
NB600-531 in the following applications:

Reactivity Notes

Predicted by homology: bovine, chicken, frog, porcine, and rabbit, mouse, rat.

Alternate Names for alpha-Smooth Muscle Actin Antibody

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • anti-alpha SMA
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin


Actins are highly conserved proteins expressed in all eukaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is encoded by a separated gene and is expressed in a developmentally regulated and tissue-specific manner, alpha and beta cytoplasmic actins are expressed in a wide variety of cells; whereas, expression of alpha skeletal, alpha cardiac, alpha vascular, and gamma enteric actins are more restricted to specialized muscle cell type. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Simple Western, IHC, ICC, ICFlow
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, ICC
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ICC
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
Species: Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for alpha-Smooth Muscle Actin Antibody (NB600-531)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-FrFl, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB600-531 Applications Species
Marchi S, Corricelli M, Trapani E et al. Defective autophagy is a key feature of cerebral cavernous malformations. EMBO Mol Med 2015 Nov [PMID:26417067] (ICC/IF) ICC/IF
Ge X, Pettan-Brewer C, Morton J et al. Mitochondrial catalase suppresses naturally occurring lung cancer in old mice. Pathobiol Aging Age Relat Dis 2015 Sep 24 [PMID:26400209] (IHC, Mouse) IHC Mouse
Inada E, Saitoh I, Watanabe S et al. PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells Int J Oral Sci. 2015 Jul 24 [PMID:26208039] (ICC/IF, Human) ICC/IF Human
Kwon JJ, Nabinger SC, Vega Z et al. Pathophysiological role of microRNA-29 in pancreatic cancer stroma. Sci Rep 2015 [PMID:26095125] (ICC/IF) ICC/IF
Tate CM, Mc Entire J, Pallini R et al. A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology. PLoS ONE. 2015 Apr 29 [PMID:25919028] (IHC-FrFl, Human) IHC-FrFl Human
Coyle D, O'Donnell AM, Puri P. Altered distribution of small-conductance calcium-activated potassium channel SK3 in Hirschsprung's disease Journal of Pediatric Surgery. 2015 Feb 29 [PMID:25783396] (IHC-Fr, Human) IHC-Fr Human
Ramani K, Donoyan S, Tomasi Ml, Park S. Role of Methionine Adenosyltransferase Alpha2 and B Phosphorylation and Stabilization in Human Hepatic Stellate Cell Trans-Differentiation. J. Cell. Physiol. 2014 Oct 07 [PMID:25294683] (WB, ICC/IF, Human) WB, ICC/IF Human
Court H, Amoyel M, Hackman M et al. Isoprenylcysteine carboxylmethyltransferase deficiency exacerbates KRAS-driven pancreatic neoplasia via Notch suppression. J Clin Invest. 2013 Oct 8 [PMID:24216479] (IHC-P, Mouse) IHC-P Mouse
Moriondo A, Solari E, Marcozzi C, Negrini D. Spontaneous activity in peripheral diaphragmatic lymphatic loops. Am J Physiol Heart Circ Physiol. 2013 Oct [PMID:23893166] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Takamura H, Nakanuma S, Hayashi H et al. Evaluation of eligibility criteria in living donor liver transplantation for hepatocellular carcinoma by alpha-SMA-positive cancer-associated fibroblasts. Oncol Rep 2013 Oct [PMID:23863893] (ICC/IF, Human) ICC/IF Human
Show All 26 Publications.

Reviews for alpha-Smooth Muscle Actin Antibody (NB600-531) (0)

There are no reviews for alpha-Smooth Muscle Actin Antibody (NB600-531). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-Smooth Muscle Actin Antibody (NB600-531). (Showing 1 - 1 of 1 FAQs).

  1. I'd like to use this antibody on paraffin embedded sections. What kind of antigen retrieval should I use?
    • We did not use a customized protocol for the validation of this product and our general IHC-P protocol may be found here. The antigen retrieval method which is routinely employed in our lab is Citrate buffer based Heat Induced Epitope Retrieval (HIER) which as follows: Dissolve 1.92 g of Citric acid (anhydrous) in 1000 ml of distilled water. Adjust pH to 6.0 w/ 1N NaOH and add 0.5 ml of Tween 20, mix well. Pre-heat steamer or water bath with staining dish containing Sodium Citrate Buffer or Citrate Buffer until temperature reaches 95-100 degrees Celcius. Immerse slides in the staining dish. Place the lid loosely on the staining dish and incubate for 20-40 minutes. Remove the staining dish to room temperature and allow the slides to cool for 20 minutes before proceeding with normal staining procedure. NB600-531 is one of our best sellers with nice customer feedback and citations in at least 13 peer reviewed publications in journals of high repute. In fact in many publications the authors have used this product for IHC application and you may follow the same to see what would work best for your samples and to compare your outcome. Please keep us posted with your results from the assay and kindly do not hesitate to contact us again if additional feedback is required or if you have other queries on any of our products.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional alpha-Smooth Muscle Actin Antibody Products

Related Products by Gene

Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody (NB600-531)

Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody (NB600-531). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for alpha-Smooth Muscle Actin Antibody (NB600-531)

Discover more about diseases related to alpha-Smooth Muscle Actin Antibody (NB600-531).

Pathways for alpha-Smooth Muscle Actin Antibody (NB600-531)

View related products by pathway.

PTMs for alpha-Smooth Muscle Actin Antibody (NB600-531)

Learn more about PTMs related to alpha-Smooth Muscle Actin Antibody (NB600-531).

Research Areas for alpha-Smooth Muscle Actin Antibody (NB600-531)

Find related products by research area.

Blogs on alpha-Smooth Muscle Actin.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol ACTA2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB600-531 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought