Home » alpha-Smooth Muscle Actin » alpha-Smooth Muscle Actin Antibody Pairs » alpha-Smooth Muscle Actin Antibody Pair

alpha-Smooth Muscle Actin Antibody Pair

 Submit a Review

Images

 
(
Enlarge)
ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using human uterus myoma tissue lysate as an analyte (non-denatured). Sandwich ELISA detection sensitivity ranging from 100 ng/ml to 300 ug/ml for lysate total protein.
ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using human uterus myoma tissue lysate as an analyte (non-denatured). Sandwich ELISA detection sensitivity ranging from 100 ng/ml to 300 ...read more
ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using recombinant protein ( H00000059-P01 ) as an analyte. Sandwich ELISA detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.
Sandwich ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Detection sensitivity ranging from approximately 6561x to 729x dilution of the ACTA2 293T overexpression lysate (non-denatured). Standard curve ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications S-ELISA
Clonality
Monoclonal
Standard Curve Range
0.3 ng/ml-100 ng/ml

Order Details

100% Guarantee on every product

alpha-Smooth Muscle Actin Antibody Pair Summary

Description
This antibody pair set comes with matched antibody pair to detect and quantify protein level of human ACTA2.

  • Capture antibody: mouse monoclonal anti-ACTA2 IgG2b Kappa (100 ug)
  • Detection antibody: biotinylated mouse monoclonal anti-ACTA2 IgG1 Kappa (50 ug)
    		•
    Standard Curve Range
    0.3 ng/ml-100 ng/ml
    Assay Type
    Sandwich ELISA
    Clonality
    Monoclonal
    Gene
    ACTA2
    Purity
    Immunogen affinity purified

    Applications/Dilutions

    Dilutions
    • Sandwich ELISA 0.3 ng/ml - 100 ng/ml

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    Quality control test: Standard curve using recombinant protein (H00000059-P01) as an analyte.

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for alpha-Smooth Muscle Actin Antibody Pair

    • AAT6
    • ACTA2
    • Actin alpha 2
    • actin, alpha 2, smooth muscle, aorta
    • actin, aortic smooth muscle
    • ACTSA
    • ACTVS
    • alpha 2 actin
    • alpha SMA
    • alpha smooth muscle actin
    • alpha-actin-2
    • alpha-SMA
    • alphaSmooth Muscle Actin
    • alpha-Smooth Muscle Actin
    • a-sma
    • Cell growth-inhibiting gene 46 protein
    • growth-inhibiting gene 46
    • MYMY5
    • SMA
    • smooth muscle actin

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

    Customers Who Viewed This Item Also Viewed...

    Vimentin Antibody
    NB300-223
    Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - analysis of HeLa cell culture stained with chicken pAb to vimentin, NB300-223, dilution 1:10,000 in green, and costained with EnCor mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
    Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates using chicken pAb to Vimentin, NB300-223, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
    (1 Review    )
    (22 Publications    )
    Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
    Applications: WB, ICC/IF, ICC
    Fibronectin
    Antibody
    NBP1-91258
    Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.
    Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Fibronectin at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
    Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-FR and IF (Alexa Fluor488) was performed using NBP1-91258 at 1:400 dilution. Image was captured by epifluorescent microscopy. Image from verified customer review.
    (6 Reviews    )
    (9 Publications    )
    Species: Hu, Mu, Bv, Fe
    Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
    Desmin Antibody
    NB120-15200
    Immunohistochemistry-Paraffin: Desmin Antibody [NB120-15200] - IHC analysis of Desmin in paraffin embedded and frozen E8-E11 mouse embryos. Image courtesy of product review submitted by Tara Rasmussen.
    Immunohistochemistry-Paraffin: Desmin Antibody [NB120-15200] - Human skeletal muscle stained with anti-desmin antibody.
    (1 Review    )
    (9 Publications    )
    Species: Hu, Mu, Sh
    Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
    CD34 Antibody (MEC
    14.7)
    NB600-1071
    Immunocytochemistry/Immunofluorescence: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 antibody was tested in WEHI-3 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
    Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) [NB600-1071] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse small intestine using rat anti-mouse CD34 (clone MEC 14.7) at 1:100 dilution. The signal was developed using HRP-conjugated anti-rat secondary with DAB reagent which followed counterstaining of nuclei using hematoxylin. This antibody specifically labelled the endothelial cells in blood vessels located primarily in the sub-mucosa, and of that of the mucosa muscularis and the mucosal lacteal.
    Flow Cytometry: CD34 Antibody (MEC 14.7) [NB600-1071] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black).
    (17 Publications    )
    Species: Mu, Rt
    Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
    CTGF/CCN2 Antibody
    NB100-724
    Immunocytochemistry/Immunofluorescence: CTGF/CCN2 Antibody [NB100-724] - CTGF antibody was tested in U2OS cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
    (4 Publications    )
    Species: Hu, Mu, Rt
    Applications: WB, ICC/IF, IHC
    NOD2 Antibody (2D9)
    NB100-524
    Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
    Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
    Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
    (1 Review    )
    (13 Publications    )
    Species: Hu, Mu
    Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
    CTLA-4 Antibody
    [Unconjugated]
    AF-386-PB
    Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA‑4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
    CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
    (2 Reviews    )
    (2 Publications    )
    Species: Hu
    Applications: WB, Flow, CyTOF-ready, ICC
    HLA DQA1 Antibody
    NBP1-84550
    Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.
    Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
    Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
    Species: Hu
    Applications: WB, IHC, IHC-P
    E-Cadherin
    Antibody [Unconjugate
    AF748
    Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, P19 mouse embryonal carcinoma cell line, and 4T1 mouse breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for E‑Cadherin at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    Simple Western lane view shows lysates of 4T1 mouse breast cancer cell line, P19 mouse embryonal carcinoma cell line, A431 human epithelial carcinoma cell line, and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for E‑Cadherin at approximately 128 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse E‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
    E‑Cadherin was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human/MouseE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF748) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
    (5 Reviews    )
    (23 Publications    )
    Species: Hu, Mu
    Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
    FAK Antibody
    (OTI4D11)
    NBP2-45923
    Western Blot: FAK Antibody (4D11) [NBP2-45923] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAK.
    Immunocytochemistry/Immunofluorescence: FAK Antibody (OTI4D11) [NBP2-45923] - FAK Antibody (4D11) [NBP2-45923] - Analysis of HeLa cells.
    Immunohistochemistry: FAK Antibody (4D11) [NBP2-45923] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
    Species: Hu
    Applications: WB, ICC/IF, IHC, IHC-P
    Smad2/3 Antibody
    [Unconjugated]
    AF3797
    Western blot shows lysates of A549 human lung carcinoma cell line, Jurkat human acute T cell leukemia cell line, HEK293 human embryonic kidney cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human Smad2 and Smad3 were included. Specific bands for Smad2 were detected at approximately 64 and 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
    Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Smad2/3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) or control antibody (Catalog # AB‑108‑C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The c-myc promoter was detected by standard PCR.
    Simple Western lane view shows lysates of A549 human lung carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and HT‑29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad2/3 at approximately 64 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse Smad2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3797) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
    (2 Reviews    )
    (8 Publications    )
    Species: Hu, Mu
    Applications: WB, Simple Western, ChIP, ICC
    FGF basic/FGF2
    [Unconjugated]
    233-FB/CF
    1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
    Recombinant Human FGF basic (146aa) (Catalog # 233-FB/CF) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
    (231 Publications    )
    Species: Hu
    SLC22A3 Antibody
    NBP1-83976
    Immunocytochemistry/Immunofluorescence: SLC22A3 Antibody [NBP1-83976] - Immunofluorescent staining of human cell line A549 shows localization to cytosol & vesicles.
    Immunohistochemistry-Paraffin: SLC22A3 Antibody [NBP1-83976] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
    Species: Hu
    Applications: ICC/IF, IHC, IHC-P
    TGF-beta RI/ALK-5
    Antibody [Unconjugate ...
    AF3025
    Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TGF‑ beta  RI/ALK‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TGF‑ beta  RI/ALK‑5 at approximately 50 & 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    TGF-beta  RI/ALK‑5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Goat Anti-Human TGF‑ beta  RI/ALK‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3025) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
    (4 Publications    )
    Species: Hu
    Applications: WB, ELISA, ICC
    ITK Antibody (5G6)
    NBP2-37578
    Western Blot: ITK Antibody (5G6) [NBP2-37578] - Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
    Immunocytochemistry/Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
    Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
    Species: Hu
    Applications: WB, ELISA, Flow, ICC/IF
    CD31/PECAM-1
    Antibody [Unconjugate
    AF3628
    Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    CD31/PECAM‑1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing endothelium. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
    Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
    (8 Reviews    )
    (38 Publications    )
    Species: Mu, Rt
    Applications: WB, Flow, IHC, CyTOF-ready, ICC

    Publications for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41) (0)

    There are no publications for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41).
    By submitting your publication information earn gift cards and discounts for future purchases.

    Reviews for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41) (0)

    There are no reviews for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
    • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
    • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
    • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

    FAQs for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41) (0)

    There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

    Additional alpha-Smooth Muscle Actin Products

    Bioinformatics Tool for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41)

    Discover related pathways, diseases and genes to alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
    Visit Tool

    Diseases for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41)

    Discover more about diseases related to alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41).
    		 

    Pathways for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41)

    View related products by pathway.

    PTMs for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41)

    Learn more about PTMs related to alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41).
    		 

    Research Areas for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41)

    Find related products by research area.

    Blogs on alpha-Smooth Muscle Actin.

    Check out the latest blog posts on alpha-Smooth Muscle Actin.
    Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
    Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.
    Understanding Actin Alpha 2 Smooth Muscle
    Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

    Customers Who Bought This Also Bought

    SDS-Page: Recombinant Human alpha-Smooth Muscle Actin Protein [NBP2-22670]
    Recombinant Human alpha-Smooth Muscle Actin Protein
    NBP2-22670
    1 µg/lane of Recombinant Human FGF basic (146 aa) was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
    FGF basic/FGF2 [Unconjugated]
    233-FB-025/CF
    Western Blot: alpha-Smooth Muscle Actin Antibody [NB300-978] - (0.1ug/ml) staining of Human Duodenum (A) and Heart (B) lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    alpha-Smooth Muscle Actin Antibody
    NB300-978
    Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3628) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD31/PECAM‑1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
    CD31/PECAM-1 Antibody [Unconjugated]
    AF3628

    Contact Information

    Product PDFs

      PDF Datasheet
      Request CofA
      Request SDS

    Review this Product

    Be the first to review our alpha-Smooth Muscle Actin Antibody Pair and receive a gift card or discount.


    Bioinformatics

    Gene Symbol ACTA2
    Entrez