alpha-Smooth Muscle Actin Antibody Pair

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using human uterus myoma tissue lysate as an analyte (non-denatured). Sandwich ELISA detection sensitivity ranging from 100 ng/ml to 300 ug/ml for lysate total protein.

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using recombinant protein ( H00000059-P01 ) as an analyte. Sandwich ELISA detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.

Sandwich ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Detection sensitivity ranging from approximately 6561x to 729x dilution of the alpha-Smooth Muscle Actin 293T overexpression lysate (non-denatured). Standard curve using alpha-Smooth Muscle Actin 293T overexpression lysate (non-denatured) as an analyte.

• Reactivity: Hu
• Applications: S-ELISA
• Clonality: Monoclonal
• Standard Curve Range: 0.3 ng/ml-100 ng/ml

alpha-Smooth Muscle Actin Antibody Pair Summary

• Description: This antibody pair set comes with matched antibody pair to detect and quantify protein level of human ACTA2.
  
  
• Capture antibody: mouse monoclonal anti-ACTA2 IgG2b Kappa (100 ug)
• Detection antibody: biotinylated mouse monoclonal anti-ACTA2 IgG1 Kappa (50 ug)
• Standard Curve Range: 0.3 ng/ml-100 ng/ml
• Assay Type: Sandwich ELISA
• Clonality: Monoclonal
• Gene: ACTA2

Applications/Dilutions

• Dilutions:
  • Sandwich ELISA 0.3 ng/ml - 100 ng/ml
• Application Notes: Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 1x PBS, pH 7.4

Notes

Quality control test: Standard curve using recombinant protein (H00000059-P01) as an analyte.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for alpha-Smooth Muscle Actin Antibody Pair

• AAT6
• ACTA2
• Actin alpha 2
• actin, alpha 2, smooth muscle, aorta
• actin, aortic smooth muscle
• ACTSA
• ACTVS
• alpha 2 actin
• alpha SMA
• alpha smooth muscle actin
• alpha-actin-2
• alpha-SMA
• alphaSmooth Muscle Actin
• alpha-Smooth Muscle Actin
• a-sma
• Cell growth-inhibiting gene 46 protein
• growth-inhibiting gene 46
• MYMY5
• SMA
• smooth muscle actin

Background

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

FAQs for alpha-Smooth Muscle Actin Antibody Pair (H00000059-AP41). (Showing 1 - 1 of 1 FAQ).

1. I do not see horse listed as a predicted or confirmed species. Would you be able to confirm if any of your alpha-SMA antibodies will react with equine samples?
   • We have not directly tested equine samples nor do we have cited publications that have tested equine samples. Since alpha-Smooth Muscle Actin is a highly conserved sequence, these antibodies should react with most mammal species.

Bioinformatics

• Gene Symbol: ACTA2