alpha-Smooth Muscle Actin Antibody (0.N.5)

Images

 
Immunohistochemistry-Paraffin: alpha-Smooth Muscle Actin Antibody (0.N.5) [NB120-18147] - Formalin-fixed, paraffin-embedded human leiomyoma using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staning of smoth ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bb, Ch, RbSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clone
0.N.5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.04 mg/ml

Order Details

alpha-Smooth Muscle Actin Antibody (0.N.5) Summary

Immunogen
N-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. The specific eptiope is the Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth Actin. Cellular Localization: Cytoplasmic
Epitope
Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth actin.
Localization
Cytoplasmic
Marker
Mesenchymal Cell Marker
Specificity
Recognizes the alpha-smooth muscle isoform of actin. Does not react with actin from fibroblasts (b-and g-cytoplasmic), striated muscle (a-sarcomeric), and myocardium (a-myocardial).
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ACTA2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:400
Positive Control
alpha-Actinin 1 Lysate (NBL1-07282)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 0.2% BSA
Preservative
0.09% Sodium Azide
Concentration
0.04 mg/ml
Purity
Protein A purified

Alternate Names for alpha-Smooth Muscle Actin Antibody (0.N.5)

  • AAT6
  • ACTA2
  • Actin alpha 2
  • actin, alpha 2, smooth muscle, aorta
  • actin, aortic smooth muscle
  • ACTSA
  • ACTVS
  • alpha 2 actin
  • alpha SMA
  • alpha smooth muscle actin
  • alpha-actin-2
  • alpha-SMA
  • alphaSmooth Muscle Actin
  • alpha-Smooth Muscle Actin
  • a-sma
  • Cell growth-inhibiting gene 46 protein
  • growth-inhibiting gene 46
  • MYMY5
  • SMA
  • smooth muscle actin

Background

Stains smooth muscle cells in vessel walls, gut walls, and myometrium. Myoepithelial cells in breat and salivary gland are also stained. This antibody reacts with tumors arising from smooth muscles and myoepithelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for alpha-Smooth Muscle Actin Antibody (NB120-18147) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on alpha-Smooth Muscle Actin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Understanding Actin Alpha 2 Smooth Muscle
Actins are extremely highly conserved structural proteins found in all eukaryotic cell cytoskeletons that govern cell structure, movement, and shape integrity. Six distinct actin isoforms, each encoded by a different gene and developmentally-regulated...  Read full blog post.

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Bioinformatics

Gene Symbol ACTA2
Entrez