alpha-Internexin Antibody

Images

 
Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot of extract of rat brain stem crude extract stained with NBP1-05208, showing a single strong clean band at approx. 66kDa.
Immunocytochemistry/ Immunofluorescence: alpha-Internexin Antibody [NBP1-05208] - Mixed neuron glia cultures stain with NBP1-05208 (red) and counterstained with RPCA-Cor1a, EnCor's rabbit polyclonal antibody to Coronin ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

alpha-Internexin Antibody Summary

Immunogen
Rat alpha Internexin [UniProt# P23565]
Marker
Immature Neuronal Marker
Isotype
IgY
Clonality
Polyclonal
Host
Chicken
Gene
INA
Purity
Ammonium sulfate precipitation
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
Application Notes
This alpha Internexin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot In Western blot a band is observed at either 66 or 68 kDa in brain or spinal cord homogenate. The human protein runs at ~68kDa, while rat, mouse and other species run at ~66kDa. Minor bands at ~150kDa are probably covalent dimers, and bands at ~50kDa represent a-internexin breakdown products. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Ammonium sulfate precipitation

Alternate Names for alpha-Internexin Antibody

  • 66 kDa neurofilament protein
  • alphaInternexin
  • alpha-Internexin
  • alpha-Inx
  • FLJ18662
  • FLJ57501
  • INA
  • internexin neuronal intermediate filament protein, alpha
  • MGC12702
  • NEF5
  • neurofilament 5 (66kD)
  • Neurofilament 5
  • Neurofilament-66
  • neurofilament-66, tax-binding protein
  • NF-66
  • NF-66alpha-internexin
  • TXBP-1

Background

In mammalian CNS, five key neuronal intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, INA or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functionally associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to DNA damage and has been associated with developmental, degenerative /overexpression causes neuronal cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological signature of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neuronal intermediate filament inclusion disease (NIFID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol INA
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