alpha-Internexin Antibody

Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot analysis of different tissue lysates using chicken pAb to alpha-Internexin, CPCA-a-Int, dilution 1:10,000 in green: (1) protein standard (red), (2) rat brain, (3) rat spinal cord, (4) mouse brain, (5) mouse spinal cord, (6) cow spinal cord and (7) pig spinal cord. CPCA-a-Int antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody [NBP1-05208] - Mixed neuron glia cultures stain with NBP1-05208 (red) and counterstained with RPCA-Cor1a, EnCor's rabbit polyclonal antibody to Coronin 1a, (green) which is an excellent marker of microglia and lymphocytes. The chicken internexin antibody is an excellent marker of neuronal processes in these cultures.

Immunohistochemistry Free-Floating: alpha-Internexin Antibody [NBP1-05208] - Analysis of a rat cerebellum section stained with chicken alpha-Internexin pAb , dilution 1:5,000 (red), and costained with mouse MBP mAb, 1:5,000 (Green). Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The alpha-Internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.

Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot of extract of rat brain stem crude extract stained with NBP1-05208, showing a single strong clean band at approx. 66kDa.

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq
• Applications: WB, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

alpha-Internexin Antibody Summary

• Description: Novus Biologicals Chicken alpha-Internexin Antibody (NBP1-05208) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified full length recombinant rat alpha-internexin expressed in and purified from E. coli.
• Marker: Immature Neuronal Marker
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: INA
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000
  • Immunohistochemistry Free-Floating 1:1000
  • Immunohistochemistry 1:1000
  • Western Blot 1:10000
• Application Notes: This alpha Internexin antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, and Immunohistochemistry. In Western blot a band is observed at either 66 or 68 kDa in brain or spinal cord homogenate. The human protein runs at ~68kDa, while rat, mouse and other species run at ~66kDa. Minor bands at ~150kDa are probably covalent dimers, and bands at ~50kDa represent a-internexin breakdown products.
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
• Preservative: 0.035% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for alpha-Internexin Antibody

• 66 kDa neurofilament protein
• alphaInternexin
• alpha-Internexin
• alpha-Inx
• FLJ18662
• FLJ57501
• INA
• internexin neuronal intermediate filament protein, alpha
• MGC12702
• NEF5
• neurofilament 5 (66kD)
• Neurofilament 5
• Neurofilament-66
• neurofilament-66, tax-binding protein
• NF-66
• NF-66alpha-internexin
• TXBP-1

Background

In mammalian CNS, five key neuronal intermediate filament (NIF) proteins have been discovered which includes alpha-internexin (66 kD, INA or Inexa), peripherin (57 kD), and three neurofilament (NF) proteins, which are neurofilament light (NF-L, 68 kD), medium (NF-M, 145 kD), and heavy (NF-H, 200 kD). Among NIFs, alpha-internexin is categorized as class IV NIF which is highly expressed in most neurons during development especially when they begin to differentiate and before the expression of NF triplet proteins, and it is structurally/functionally associated with NF triplet proteins inside mature CNS neurons. Peripherin is mainly expressed in PNS and in some CNS neurons also, and alpha-internexin - peripherin self-assemble or co-assemble with neurofilament protein subunits to form filamentous structure before their translocation into the axons to become an essential constituent of shape-maintaining IF network in mature neurons. It has been recognized as one of the many proteins phosphorylated by ATM/ATR in response to DNA damage and has been associated with developmental, degenerative /overexpression causes neuronal cell death, and inflammatory processes of CNS. Abnormal NIF accumulation is a neuropathological signature of several neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, dementia with Lewy bodies, and amyotrophic lateral sclerosis. Alpha-internexin has been detected as a major component of pathological inclusions in frontotemporal dementia, also called 'neuronal intermediate filament inclusion disease (NIFID).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INA
• Entrez:
  • 24503(Rat)