alpha-Internexin Antibody

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, human, mouse, and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat a-Internexin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4844) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for alpha-Internexin at approximately 66 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-Internexin Antibody Summary

• Immunogen: E. coli-derived recombinant human alpha -Internexin
  Val230-Glu450
  Accession # Q16352
• Specificity: Detects endogenous human, mouse, and rat alpha -Internexin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: INA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Internexin Antibody

• 66 kDa neurofilament protein
• alphaInternexin
• alpha-Internexin
• alpha-Inx
• FLJ18662
• FLJ57501
• INA
• internexin neuronal intermediate filament protein, alpha
• MGC12702
• NEF5
• neurofilament 5 (66kD)
• Neurofilament 5
• Neurofilament-66
• neurofilament-66, tax-binding protein
• NF-66
• NF-66alpha-internexin
• TXBP-1

Background

Alpha-Internexin ( alpha -INX; also 66 kDa neurofilament protein (NF-66)) is a 66 kDa member of the intermediate filament (IF) protein family. It was the first of two molecules named for its presumed interaction with cytoskeletal proteins. alpha -INX is one of four Class IV neuronal IF proteins. It both self-assembles, and complexes with NF-L, H and M in select cortical and cerebellar neurons. Human alpha -INX is 499 amino acids (aa) in length. It has a predicted MW of 55 kDa, but runs anomalously at 66 kDa in SDS-PAGE. alpha -INX contains one DNA-binding region (aa 10‑92), a poly-Glu segment (aa 449‑454) and three serine phosphorylation sites (Ser72/335/496). There is one 494 aa alternative splice form that shows multiple short sequence aa substitutions in the first 200 amino acids. Over aa 230‑450, human alpha -INX shares more that 96% aa identity with mouse, canine and rat alpha -INX.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INA
• Uniprot:
  • Q16352(Human)