alpha-Internexin Antibody (JU25-03)

Western Blot: alpha-Internexin Antibody (JU25-03) [NBP2-75407] - Western blot analysis of alpha-Internexin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: A549 cell lysate Lane 1: mouse brain tissue lysate Lane 2: PC-12 cell lysate

Immunocytochemistry/Immunofluorescence: alpha-Internexin Antibody (JU25-03) [NBP2-75407] - Staining alpha Internexin in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: alpha-Internexin Antibody (JU25-03) [NBP2-75407] - Analysis of paraffin-embedded rat brain tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin.

Flow Cytometry: alpha-Internexin Antibody (JU25-03) [NBP2-75407] - Analysis of M2A cells with alpha Internexin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: alpha-Internexin Antibody (JU25-03) [NBP2-75407] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin.

Product Discontinued

alpha-Internexin Antibody (JU25-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human alpha-Internexin aa 394-443 / 499. (SwissProt: Q16352 Human; SwissProt: P46660 Mouse; SwissProt: P23565 Rat)
• Localization: Intermediate filament cytoskeleton, neurofilament, extracellular space, cytoplasmic ribonucleoprotein granule, postsynapse, Schaffer collateral - CA1 synapse.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:5000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for alpha-Internexin Antibody (JU25-03)

• 66 kDa neurofilament protein
• alphaInternexin
• alpha-Internexin
• alpha-Inx
• FLJ18662
• FLJ57501
• INA
• internexin neuronal intermediate filament protein, alpha
• MGC12702
• NEF5
• neurofilament 5 (66kD)
• Neurofilament 5
• Neurofilament-66
• neurofilament-66, tax-binding protein
• NF-66
• NF-66alpha-internexin
• TXBP-1

Background

Alpha-internexin is a Class IV intermediate filament, related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. However, many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H, and some mature neurons only express the alpha-internexin subunit of neurofilament.

The very early developmental expression of alpha-internexin suggests that its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. Therefore, alpha-internexin antibodies serve as unique probes to study and classify neuronal types and follow their processes in tissue sections and culture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INA