alpha Adducin Antibody - BSA Free

Western Blot: alpha Adducin Antibody [NBP2-15359] - A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 7.5 % SDS-PAGE alpha Adducin antibody [N1N2], N-term dilution: 1:1000

Immunohistochemistry-Paraffin: alpha Adducin Antibody [NBP2-15359] - Paraffin-embedded SG xenograft, using antibody at 1:100 dilution.

Western Blot: alpha Adducin Antibody [NBP2-15359] - Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry: alpha Adducin Antibody [NBP2-15359] - alpha Adducin antibody [N1N2], N-term detects alpha Adducin protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. alpha Adducin antibody [N1N2], N-term (NBP2-15359) dilution: 1:200.

• Reactivity: Hu, Mu, Ze, Rt, Bv, Ch, RM, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

alpha Adducin Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Adducin. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton; Cell membrane
• Predicted Species: Rat (98%), Chicken (98%), Xenopus (93%), Bovine (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADD1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%), Xenopus laevis (93%), Zebrafish (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for alpha Adducin Antibody - BSA Free

• ADDA
• adducin 1 (alpha)
• alpha-adducin
• erythrocyte adducin alpha subunit
• Erythrocyte adducin subunit alpha
• MGC3339
• MGC44427

Background

Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ADD1
• Uniprot:
  • P35611(Mouse)