Alpha Actinin 4 Antibody (RM287)

Western Blot: Alpha Actinin 4 Antibody (RM287) [NBP2-61485] - Western Blot of HeLa cells lysates using NBP2-61485 at a 1:1000 dilution.

Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (RM287) [NBP2-61485] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using NBP2-61485 at a 1:200 dilution

• Reactivity: Hu
• Applications: WB, IHC
• Clone: RM287
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Alpha Actinin 4 Antibody (RM287) Summary

• Description: Novus Biologicals Rabbit Alpha Actinin 4 Antibody (RM287) (NBP2-61485) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide corresponding to the N-terminus of Human Alpha Actinin 4
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACTN4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:200
  • Immunohistochemistry-Paraffin 1:100-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Alpha Actinin 4 Antibody (RM287)

• actinin alpha4 isoform
• actinin, alpha 4
• ACTININ-4
• alpha-actinin-4
• DKFZp686K23158
• F-actin cross-linking protein
• focal segmental glomerulosclerosis 1
• FSGS
• FSGS1
• Non-muscle alpha-actinin 4

Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTN4