alpha 2u-Globulin Antibody (129736) [Biotin]

a2u-Globulin was detected in perfusion fixed frozen sections of rat liver using Mouse Anti-Rat a2u-Globulin Biotinylated Monoclonal Antibody (Catalog # BAM586) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

alpha 2u‑Globulin was detected in immersion fixed paraffin-embedded sections of rat kidney using Mouse Anti-Rat alpha 2u‑Globulin Biotinylated Monoclonal Antibody (Catalog # BAM586) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Rt
• Applications: WB, IHC
• Clone: 129736
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

alpha 2u-Globulin Antibody (129736) [Biotin] Summary

• Immunogen: Rat urine-derived alpha _2u-Globulin
• Specificity: Detects rat alpha _2u-Globulin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2u-Globulin Antibody (129736) [Biotin]

• alpha 2u-Globulin
• AUG
• Mup
• PGCL1

Background

alpha _2u-Globulin, also known as PGCL1, is a member of the Lipocalin family that show 87-98% amino acid sequence identity. alpha _2u-Globulin is the major urinary protein excreted by adult male rat. It is expressed at highest levels in male liver and coagulate glands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2u-Globulin Antibody (BAM586)(1)

Publication using BAM586

• MOROKI T, MATSUO S, HATAKEYAMA H et al. Databases for technical aspects of immunohistochemistry-2021 update Journal of Toxicologic Pathology 2/24/2021 [PMID: 33976473]