alpha 2u-Globulin Antibody (129736) [Unconjugated]

a2u-Globulin was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat a2u-Globulin Monoclonal Antibody (Catalog # MAB586) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Rt
• Applications: IHC
• Clone: 129736
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

alpha 2u-Globulin Antibody (129736) [Unconjugated] Summary

• Immunogen: Rat urine-derived alpha _2u-Globulin
• Specificity: Detects rat alpha _2μ-Globulin in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha 2u-Globulin Antibody (129736) [Unconjugated]

• alpha 2u-Globulin
• AUG
• Mup
• PGCL1

Background

alpha -_2u Globulin, also known as PGCL1, is a member of a highly homologous multigene family of more than 20 members that show 87%‑98% amino acid identity. It is expressed at highest levels in male liver and coagulate glands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for alpha 2u-Globulin Antibody (MAB586)(1)

Publication using MAB586

• Kerachian MA, Cournoyer D, Harvey EJ, Chow TY, Begin LR, Nahal A, Seguin C New insights into the pathogenesis of glucocorticoid-induced avascular necrosis: microarray analysis of gene expression in a rat model. Arthritis Res. Ther., 2010-06-25;12(3):R124. 2010-06-25 [PMID: 20579363] (IHC-P, Rat)