MUP-1 Antibody (663323)

MUP-1 was detected in immersion fixed NCTC 1469 mouse liver cell line using Mouse MUP-1 Monoclonal Antibody (Catalog # MAB6560) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Mu
• Applications: ICC/IF
• Clone: 663323
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MUP-1 Antibody (663323) Summary

• Immunogen: Mouse MUP-1 synthetic peptide
  Accession # NP_112465
• Specificity: Detects mouse MUP-1 in direct ELISAs.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: Mup1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MUP-1 Antibody (663323)

• Ltn-1
• Lvtn-1
• major urinary protein 1
• major urinary protein 10
• major urinary protein 7
• MUP 1
• Mup1 major urinary protein 1
• MUP1
• MUP-1
• Mup10
• Mup7
• Mup-a
• Up-1

Background

Major urinary protein 1 (MUP-1) is a 20 kDa lipocalin family glycoprotein that is secreted by the liver into the circulation and then excreted in the urine of male mice. Its beta -barrel structure with an internal hydrophobic cavity enables MUP-1 to serve as a carrier for a variety of pheromones. In the circulation, MUP-1 promotes energy expenditure, mitochondrial biogenesis, and oxidative function, and it inhibits hepatic glucose production, triglyceride accumulation, glucose intolerance, and insulin resistance. MUP-1 is downregulated in the liver and circulation of diabetic obese mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUP-1 Antibody (MAB6560)(1)

Publication using MAB6560

• Tiffany A. Katz, Sandra L. Grimm, Akhilesh Kaushal, Jianrong Dong, Lindsey S. Treviño, Rahul K. Jangid et al. Hepatic Tumor Formation in Adult Mice Developmentally Exposed to Organotin Environmental Health Perspectives 2020-01-16 [PMID: 31939706]

Bioinformatics

• Gene Symbol: Mup1
• Uniprot:
  • NP_112465()