Aldolase A Antibody

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Western Blot: Aldolase A Antibody [NB600-915] - Analysis using Biotin conjugate of NB600-915. 300 ul aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and ...read more
Western Blot: Aldolase A Antibody [NB600-915] - Figure. Immunoblotting. IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate ...read more
Western Blot: Aldolase A Antibody [NB600-915] - 300 ul aliquots of whole anti-aldolase antiserum were used to precipitate varying amounts of purified aldolase and precipitates with controls were compared by SDS-PAGE and ...read more
Western Blot: Aldolase A Antibody [NB600-915] - IgG purified antibody to rabbit muscle aldolase was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived ...read more
Immunoprecipitation: Aldolase A Antibody [NB600-915] - Analysis using the Biotin conjugate of NB600-915. Detection of 300 ul of anti aldolase antiserum and an equal volume of varied amounts (diluted from a stock ...read more

Product Details

Summary
Reactivity Hu, Mu, RbSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

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Catalog# & Conjugate Size Price

Aldolase A Antibody Summary

Description
This antibody was prepared from monospecific antiserum by a delipidation, salt fractionation and ion exchange chromatography.
Immunogen
Aldolase from rabbit muscle
Localization
Cytoplasmic
Specificity
This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALDOA
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:5000
  • ELISA 1:5000 - 1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Aldolase [Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:10,000 - 1:40,000 is suggested for this product. use approximately 5 ul of antibody - immunoprecipitate 50 ul of protein lysate.
Positive Control
Aldolase A Lysate (NBL1-07459)
Publications
Read Publication using
NB600-915 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, Rabbit and Mouse. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Ion exchange chromatography

Alternate Names for Aldolase A Antibody

  • ALDA
  • aldolase A, fructose-bisphosphate
  • EC 4.1.2.13
  • fructose-1,6-bisphosphate triosephosphate-lyase
  • fructose-bisphosphate aldolase A
  • GSD12
  • Lung cancer antigen NY-LU-1
  • MGC10942
  • MGC17716
  • MGC17767
  • Muscle-type aldolase

Background

This gene product, Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aldolase A Antibody (NB600-915)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Video Protocols

WB Video Protocol

FAQs for Aldolase A Antibody (NB600-915). (Showing 1 - 1 of 1 FAQ).

  1. We have to store reference NB600-915 over the weekend and I was wondering if the storage conditions (Store at 4C short term), do apply for such a time or would we be better off putting the antibody to -20C?.
    • The antibody can be stored at 4C over the weekend as it contains sodium azide which is a stabilizer. We recommend -20C for long term storage.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ALDOA
Entrez