Aldolase A Antibody (3F9) - BSA Free

Western Blot: Aldolase A Antibody (3F9) [NBP2-42620] - The Recombinant Human ALDOA and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human ALDOA Lane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3F9) [NBP2-42620] - Analysis of ALDOA in A549 cells, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human A549 antibody (1:200) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Flow Cytometry: Aldolase A Antibody (3F9) [NBP2-42620] - An intracellular stain was performed on HeLa cells with Aldolase A Antibody (3F9)NBP2-42620AF488 and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: Aldolase A Antibody (3F9) [NBP2-42620] - Analysis of ALDOA in A549 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Aldolase A Antibody (3F9) - BSA Free Summary

• Description: Novus Biologicals Mouse Aldolase A Antibody (3F9) - BSA Free (NBP2-42620) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. Anti-Aldolase A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Aldolase A (1-364aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDOA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:32681081).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Aldolase A Antibody (3F9) - BSA Free

• ALDA
• aldolase A, fructose-bisphosphate
• EC 4.1.2.13
• fructose-1,6-bisphosphate triosephosphate-lyase
• fructose-bisphosphate aldolase A
• GSD12
• Lung cancer antigen NY-LU-1
• MGC10942
• MGC17716
• MGC17767
• Muscle-type aldolase

Background

Aldolase A (fructose bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3 phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldolase A Antibody (NBP2-42620)(1)

Publication using NBP2-42620

• Cheung KCP, Fanti S, Mauro C et al. Preservation of microvascular barrier function requires CD31 receptor-induced metabolic reprogramming Nat Commun 2020-07-17 [PMID: 32681081] (ICC/IF, Human, Mouse)
  
  Details:
  Citation using the PE/Cy7 format of this antibody.

Bioinformatics

• Gene Symbol: ALDOA
• Uniprot:
  • NP_908930(Human)