Aldolase A Antibody (3C12-6D11) - Azide and BSA Free

Western Blot: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - Analysis of ALDOA expression in transfected 293T cell line by ALDOA monoclonal antibody (M02), clone 3C12-6D11.Lane 1: ALDOA transfected lysate(39 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - Analysis of monoclonal antibody to ALDOA on A-431 cell. Antibody concentration 10 ug/ml.

Western Blot: Aldolase A Antibody (3C12-6D11) [H00000226-M02] - ALDOA monoclonal antibody (M01), clone 3C12-6D11 Analysis of ALDOA expression in A-431.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3C12-6D11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Aldolase A Antibody (3C12-6D11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ALDOA (AAH10660.1, 1 a.a. ~ 364 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY
• Specificity: ALDOA - aldolase A, fructose-bisphosphate
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALDOA
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Aldolase A Antibody (3C12-6D11) - Azide and BSA Free

• ALDA
• aldolase A, fructose-bisphosphate
• EC 4.1.2.13
• fructose-1,6-bisphosphate triosephosphate-lyase
• fructose-bisphosphate aldolase A
• GSD12
• Lung cancer antigen NY-LU-1
• MGC10942
• MGC17716
• MGC17767
• Muscle-type aldolase

Background

This gene product, Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants which encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aldolase A Antibody (H00000226-M02)(2)

Publications using H00000226-M02

• Huang Z, Hua Y, Tian Y et al. High expression of fructose-bisphosphate aldolase A induces progression of renal cell carcinoma. Oncol Rep 2018-06-01 [PMID: 29693182] (Human)
• Perroud B, Ishimaru T, Borowsky AD, Weiss RH. Grade-dependent proteomic characterization of kidney cancer. Mol Cell Proteomics. 2009-01-21 [PMID: 19164279]

Bioinformatics

• Gene Symbol: ALDOA
• Entrez:
  • 226(Human)
• OMIM:
  • 103850(Human)
• Uniprot:
  • AAH10660(Human)