ALCAM/CD166 Antibody (4H9A5) - BSA Free

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced). This image was submitted via customer Review.

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of prostate cancer tissues using ALCAM mouse mAb with DAB staining.

Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red).

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate.

Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of bladder cancer tissues using ALCAM mouse mAb with DAB staining.

ELISA: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready, KO
• Clone: 4H9A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ALCAM/CD166 Antibody (4H9A5) - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Mouse ALCAM/CD166 Antibody (4H9A5) - BSA Free (NBP2-37345) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human ALCAM/CD166 (AA: 48-216) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALCAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Knockout Validated
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 65.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ALCAM/CD166 Antibody (4H9A5) - BSA Free

• activated leucocyte cell adhesion molecule
• activated leukocyte cell adhesion moleculeFLJ38514
• ALCAM
• CD166 antigen
• CD166
• MEMDMGC71733

Background

Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic andhomophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as ininteractions between cells of the nervous system

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for ALCAM/CD166 Antibody (NBP2-37345) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Chase Taylor WB Human 03/03/2017

Summary

• Application: Western Blot
• Sample Tested: HT1080 human fibrosarcoma cell line
• Species: Human
• Lot: 140117

Comments

• Comments: Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout.
  
  Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions:
  1. Denatured, deglycosylated, reduced;
  2. Denatured;
  3. Denatured, reduced.
  
  Loading order:
  1. Protein Ladder
  2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced)
  3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced)
  4. Protein Ladder
  5. HT1080 ALCAM-RFP (Denatured)
  6. HT1080 ALCAM-KO (Denatured)
  7. Protein Ladder
  8. HT1080 ALCAM-RFP (Denatured, reduced)
  9. HT1080 ALCAM-KO (Denatured, reduced)

Bioinformatics

• Gene Symbol: ALCAM