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ALCAM/CD166 Antibody (4H9A5)

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Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions: 1. Denatured, deglycosylated, reduced; 2. Denatured; 3. Denatured, reduced. Loading order: 1. Protein Ladder 2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced) 3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced) 4. Protein Ladder 5. HT1080 ALCAM-RFP (Denatured) 6. HT1080 ALCAM-KO (Denatured) 7. Protein Ladder 8. HT1080 ALCAM-RFP (Denatured, reduced) 9. HT1080 ALCAM-KO (Denatured, reduced). This image was submitted via customer Review.
Genetic Strategies: Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Tested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout. Whole cell lysate ...read more
Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of prostate cancer tissues using ALCAM mouse mAb with DAB staining.
Flow Cytometry: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red).
Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)
Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate.
Immunohistochemistry-Paraffin: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Analysis of bladder cancer tissues using ALCAM mouse mAb with DAB staining.
ELISA: ALCAM/CD166 Antibody (4H9A5) [NBP2-37345] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, KO
Clone
4H9A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

    

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ALCAM/CD166 Antibody (4H9A5) Summary

Immunogen
Purified recombinant fragment of human ALCAM (AA: 48-216) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ALCAM
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • CyTOF-ready
  • Knockout Validated
Application Notes
This antibody is Cytof ready.
Theoretical MW
65.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-37345 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for ALCAM/CD166 Antibody (4H9A5)

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALCAM/CD166 Antibody (NBP2-37345) (0)

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Review for ALCAM/CD166 Antibody (NBP2-37345) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-37345:
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Images Ratings Applications Species Date Details
Western Blot ALCAM/CD166 NBP2-37345
Enlarge 		5
reviewed by:
Chase Taylor
 WB Human 03/03/2017
View

Summary

ApplicationWestern Blot
Sample TestedHT1080 human fibrosarcoma cell line
SpeciesHuman
Lot140117

Comments

CommentsTested antibody against two HT1080 samples: One sample is an RFP control and the other is an ALCAM knockout.

Whole cell lysate separated on 1D 12% PAGE gel under 3 conditions:
1. Denatured, deglycosylated, reduced;
2. Denatured;
3. Denatured, reduced.

Loading order:
1. Protein Ladder
2. HT1080 ALCAM-RFP (Denatured, deglycosylated, reduced)
3. HT1080 ALCAM-KO (Denatured, deglycosylated, reduced)
4. Protein Ladder
5. HT1080 ALCAM-RFP (Denatured)
6. HT1080 ALCAM-KO (Denatured)
7. Protein Ladder
8. HT1080 ALCAM-RFP (Denatured, reduced)
9. HT1080 ALCAM-KO (Denatured, reduced)

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FAQs for ALCAM/CD166 Antibody (NBP2-37345) (0)

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Western Blot ALCAM/CD166 NBP2-37345
Enlarge
 Chase Taylor
03/03/2017
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol ALCAM